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77332

Peroxidase from horseradish

Name: Peroxidase from horseradish
Brand: SIGMA
Price: 3570 CZK

lyophilized, powder, ~150 U/mg

Synonyma:

(2R,3S,6S,7R,8R)-3-(3-formamido-2-hydroxybenzamido)-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-dioxonan-7-yl 3-methylbutanoate, Antimycin A, Horseradish peroxidase

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Změnit zobrazení

Vám/SKU	Dostupnost	Cena
100 mg	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	3 570,00 Kč
500 mg	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	14 900,00 Kč

O této položce

Číslo CAS:

9003-99-0

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-668-6

MDL number:

MFCD00071339

Číslo EC:

1.11.1.7 (BRENDA, IUBMB)

eCl@ss:

32160410

Specific activity:

~150 U/mg

Biological source:

horseradish

3 570,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

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Vlastnosti

biological source

horseradish

Quality Segment

100

form

powder

quality

lyophilized

specific activity

~150 U/mg

technique(s)

protein detection: suitable

solubility

50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)

shipped in

wet ice

storage temp.

2-8°C

SMILES string

[O+H2]O[O-]

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

Popis

General description

Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.

Application

Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.

Peroxidase from horseradish has been used:

for isolation of N-Linked glycans
as a constituent of D-amino acid oxidase (DAAO) buffer
to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)

Other Notes

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C

Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater.

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Tato položka	P8415	P2088	M6908
Sigma-Aldrich 77332 Peroxidase from horseradish	Sigma-Aldrich P8415 Peroxidase from horseradish	Sigma-Aldrich P2088 Peroxidase from horseradish	Sigma-Aldrich M6908 Myeloperoxidase from human leukocytes
description lyophilized, powder, ~150 U/mg	description Type XII, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)	description Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)	description lyophilized powder, ≥50 units/mg protein
specific activity ~150 U/mg	specific activity ≥250 units/mg solid (using pyrogallol)	specific activity 200-300 units/mg solid (using pyrogallol)	specific activity ≥50 units/mg protein
biological source horseradish	biological source -	biological source horseradish	biological source human leucocytes
form powder	form essentially salt-free, lyophilized powder	form essentially salt-free, lyophilized powder	form lyophilized powder
shipped in wet ice	shipped in -	shipped in -	shipped in dry ice
solubility 50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)	solubility 0.1 M phosphate buffer: soluble (pH 6.0), H₂O: soluble	solubility -	solubility H₂O: soluble, aqueous buffers such as 50 mM sodium acetate: soluble (Freezing of the enzyme in solution may result in a substantial loss of activity.)
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C

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Piktogramy

GHS08

Výstražné slovo

Danger

Kódy nebezpečí

H334

Bezpečnostní pokyny

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Skladovací třída

13 - Non Combustible Solids

Co se děje?

WGK 1

PPE (osobní ochranné prostředky)

Eyeshields, Gloves, type N95 (US)

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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions

Anonymous

2 years ago

can we use this as an anterograde tracers to detect neuronal terminations

1 answer
1. Technical Support
  
  2 years ago
  
  This product has not been tested as an anterograde tracer to detect neuronal terminations, and no reference was found for this type of application. The products C3741 Cholera Toxin B subunit and L8777 Lectin from Phytolacca americana (pokeweed) had been referenced as neuroanatomical tracers. Below are the references:
  "Unmyelinated fibers of the anterior ethmoidal nerve in the rat co-localize with neurons in the medullary dorsal horn and ventrolateral medulla activated by nasal stimulation" by Hollandsworth MP, et al., Brain Research (2009).
  "An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: immunohistochemical localization of an axonally transported plant lectin, Phaseolus vulgaris leucoagglutinin (PHA-L)" by C R Gerfen, P E Sawchenko, Brain Research (1984-01-09).
  
  Helpful?
Anonymous

2 years ago

What is the difference between different types of peroxidase? (for instance Type I, Type II, Type IIA)

1 answer
1. Technical Support
  
  2 years ago
  
  The type designations for these enzymes are in-house designations and do not represent any kind of biochemical significance.
  
  Helpful?

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