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85878

Streptavidin from Streptomyces avidinii

affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein

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Vybrat velikost

Změnit zobrazení
Vám/SKUDostupnostCena
1 mg
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4 640,00 Kč
5 mg
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16 000,00 Kč
10 mg
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23 800,00 Kč

O této položce

Číslo CAS:
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Form:
powder
Mol wt:
Mr ~60000

4 640,00 Kč


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form

powder

Quality Segment

quality

affinity purified, lyophilized from 10 mM potassium phosphate

specific activity

≥13 U/mg protein

mol wt

Mr ~60000

solubility

H2O: 1 mg/mL, clear to hazy, colorless to faintly yellow

storage temp.

−20°C

General description

Streptavidin is a crystalline protein synthesized by Streptomycetes.

Application

Streptavidin from Streptomyces avidinii has been used:
  • in the pre-functionalization of self-propelled catalytic micromotors
  • in the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substrates
  • as an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC)

Biochem/physiol Actions

Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.
The sales quantitites 1 mg and 5 mg

Analysis Note

Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.

Other Notes

Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin; Avidin-Biotin technology
1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5

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Tato položka
S9415S2890S1638
description

affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein

description

-

description

lyophilized powder, ≥700 DEA units/mg protein

description

buffered aqueous suspension

form

powder

form

-

form

lyophilized powder

form

buffered aqueous suspension

mol wt

Mr ~60000

mol wt

-

mol wt

-

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

specific activity

≥13 U/mg protein

specific activity

-

specific activity

≥700 DEA units/mg protein

specific activity

-

solubility

H2O: 1 mg/mL, clear to hazy, colorless to faintly yellow

solubility

-

solubility

-

solubility

-

Quality Level

200

Quality Level

400, 200

Quality Level

200

Quality Level

200


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Skladovací třída

11 - Combustible Solids

Co se děje?

WGK 3

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

PPE (osobní ochranné prostředky)

Eyeshields, Gloves, type N95 (US)



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. What is the atomic mass of this Streptavidin? Is it 66 kDa?

    1 answer
    1. The atomic mass of this product is not characterized for the finished goods. However, according to the following publication, the apparent molecular weight under SDS-PAGE analysis seems to be about 66 kDa. The reference publication is AIMS Biophysics, 7(4): 236–247.

      Helpful?

  2. Is this a recombinant or native protein?

    1 answer
    1. This product is native streptavidin that has been affinity purified from fermentation cultures of Streptomyces avidinii.

      Helpful?

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