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93773

TWEEN® 20

viscosity 250-450 mPa.s (25 °C)

Synonyma:

Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

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Vám/SKUDostupnostCena
250 g
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1 190,00 Kč
1 kg
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3 700,00 Kč

O této položce

Číslo CAS:
UNSPSC Code:
12161900
NACRES:
NA.21
MDL number:

1 190,00 Kč


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description

non-ionic

Quality Segment

form

viscous liquid

mol wt

~1228

technique(s)

DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable

refractive index

n20/D 1.468 (lit.), n20/D 1.471

viscosity

250-450 mPa.s(25 °C)

HLB value

16.7±1.0

density

1.11 g/mL at 20 °C, 1.095 g/mL at 25 °C (lit.)

SMILES string

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

Non-ionic detergent
Tween® 20 is widely used in several biochemical applications. It has been used:

  • as an emulsifying agent for the preparation of stable oil-in-water emulsions
  • in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
  • as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
  • for lysing mammalian cells at a concentration of 0.005 to 0.5%
  • for rehydrating tissue before in situ hybridization

Biochem/physiol Actions

Tween® 20, functioning as a detergent, potentially operates by binding to the hydrophobic components of water-insoluble substances, thereby making them hydrophilic.

Features and Benefits

  • Suitable for protein purification and DNA and RNA extraction
  • Suitable for mammalian cell culture
  • High purity product for research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Disclaimer

may get turbid on storage, heat to clear

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Tato položka
P1379P7949P2287
description

viscosity 250-450 mPa.s (25 °C)

description

viscous liquid

description

BioXtra, viscous liquid

description

viscous liquid, suitable for cell culture

form

viscous liquid

form

viscous liquid

form

viscous liquid

form

viscous liquid

technique(s)

protein purification: suitable, RNA extraction: suitable, DNA extraction: suitable, cell culture | mammalian: suitable

technique(s)

protein purification: suitable, RNA extraction: suitable, DNA extraction: suitable, cell culture | mammalian: suitable

technique(s)

blocking: suitable, transformation: suitable, hybridization: suitable, protein purification: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

viscosity

250-450 mPa.s(25 °C)

viscosity

-

viscosity

-

viscosity

-

density

1.11 g/mL at 20 °C, 1.095 g/mL at 25 °C (lit.)

density

1.095 g/mL at 25 °C (lit.)

density

1.095 g/mL at 25 °C, 1.095 g/mL at 25 °C (lit.)

density

1.095 g/mL at 25 °C (lit.), 1.1 g/cm3 at 25 °C (lit.)

description

non-ionic

description

non-ionic

description

non-ionic

description

non-ionic

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

300


Still not finding the right product?

Explore all of our products under TWEEN® 20


Skladovací třída

10 - Combustible liquids

Bod vzplanutí (°F)

527.0 °F - Pensky-Martens closed cup

Bod vzplanutí (°C)

275 °C - Pensky-Martens closed cup

PPE (osobní ochranné prostředky)

Eyeshields, Gloves



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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