A7721
JumpStart™ Taq Antibody
Adds hot-start capabilities to any Taq DNA Polymerase
Synonyma:
Hot start Taq antibody
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O této položce
Conjugate:
unconjugated
Clone:
monoclonal
Application:
PCR, whole genome amplification
Citations:
4
Technický servis
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mouse
Quality Segment
conjugate
unconjugated
clone
monoclonal
form
liquid
feature
hotstart, PCR Additives
concentration
1.1 mg/mL
technique(s)
PCR: suitable, whole genome amplification: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
General description
JumpStart™ Taq Antibody provides an efficient method for hot-start PCR. This antibody is a neutralizing monoclonal antibody specific to Taq DNA polymerase. When the antibody is bound to Taq, the enzyme is rendered inactive. At the first denaturation step in thermal cycling, the enzyme-antibody complex dissociates, and the JumpStart Taq Antibody becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. JumpStart Taq Antibody is commonly used to prevent undesirable amplification products.
Application
JumpStart™Taq Antibody is suitable for use in:
- cytochrome blocked nucleic acid (LNA) probe assay
- ligation-mediated polymerase chain reaction (LM-PCR)
- in Real-Time quantitative PCR Analysis of viral titer
- PCR amplification of complex genomic or cDNA templates
- to amplify very low-copy-number targets
- for the PCR amplification of many thermal cycles (>35) and multiple primer pairs in the same reaction tube
Packaging
Supplied with dilution buffer.
Preparation Note
Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.
Other Notes
JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials
Dilution Buffer provided in 1 mL vials
JumpStart™ Taq Antibody is for R&D use only.Not for drug, household, or other uses.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
Skladovací třída
10 - Combustible liquids
WGK
WGK 3
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
PPE (osobní ochranné prostředky)
Eyeshields, Gloves
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