Biotin is soluble directly in water at 0.22 mg/mL, a 1 mg/mL solution is water is not achievable. However, this product is tested for solubility at 50 mg/mL in 2 N NaOH. Concentrated stock solutions prepared in NaOH may then be diluted in buffer, medium, or water to the desired concentration. The solution stability, when stored at ?20 °C, has not been investigated. The end user would have to determine stability. As a general practice, avoid repeated freeze/thaw cycles. Note that some versions of classical medium, such as Ham’s F12 (see product N6658) include D-Biotin. These sterile liquid media are stable for approximately 1 year at 2–8 °C. Similar stability would be expected for sterile Biotin stock solutions.
B4639
Biotin
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Synonyma:
D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
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Vybrat velikost
Změnit zobrazení
| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
100 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 1 700,00 Kč |
500 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 5 070,00 Kč |
1 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 8 400,00 Kč |
5 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 31 200,00 Kč |
O této položce
Empirický vzorec (Hillův zápis):
C10H16N2O3S
Číslo CAS:
Molekulová hmotnost:
244.31
NACRES:
NA.21
PubChem Substance ID:
eCl@ss:
34058011
UNSPSC Code:
12352201
EC Number:
200-399-3
MDL number:
Beilstein/REAXYS Number:
86838
1 700,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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BioReagent
Quality Segment
assay
≥99%
form
powder
mol wt
Mw 244.31 g/mol
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable
color
white to off-white
mp
231-233 °C (lit.)
solubility
H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)
storage temp.
2-8°C
SMILES string
[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
InChI
1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
InChI key
YBJHBAHKTGYVGT-ZKWXMUAHSA-N
General description
Biotin is also called as B complex vitamin. It is a bicyclic compound, which has a molecular weight of 244.31 Da. Biotin serves as an important cofactor for mammalian carboxylases. It is found within the cells in free and bound pools.
Application
Biotin has been used:
- as a M199 media supplement in in vitro culture system
- as a component of SATO serum free medium for the isolation of cortical oligodendrocyte precursors (OPCs)
- in biochemical precursors culture for de novo fatty acid synthesis
- as a component in aggregate culture media
Biochem/physiol Actions
Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.
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Tato položka | |||
|---|---|---|---|
| description powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99% | description ≥99% (HPLC), lyophilized powder | description certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland | description Pharmaceutical Secondary Standard; Certified Reference Material |
| technique(s) cell culture | insect: suitable, cell culture | plant: suitable, cell culture | mammalian: suitable | technique(s) HPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| assay ≥99% | assay ≥99% (HPLC) | assay - | assay - |
| form powder | form lyophilized powder | form - | form - |
| solubility H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.) | solubility ammonium hydroxide: 50 mg/mL (2 M) | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-30°C |
| color white to off-white | color white to off-white | color white | color - |
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Skladovací třída
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Can I dilute it in H2O for 1mg/ml stock concentration, aliquot and store at -20C?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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If I would like to make 1M biotin stock (B4639), what would be the best way?
1 answer-
A 1 M solution of biotin would require a concentration of 244 mg/mL. The maximum solubility tested for this compound is 50mg/ml in 2M NaOH or NH4OH, which results in a 204 mM solution. Similar rates have been reported for DMSO. Heat and agitation may improve the solubility. However, a precipitate may form upon cooling. A search of the literature did not reveal a more efficient solvent for Biotin. The preparation of a 1M solution would have to be determined experimentally.
Helpful?
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Hello, How can I reconstruct biotin powder (B4639)? which solvent? and how can I prepare 50mM biotin solution for 1 ml final solution?
1 answer-
This product is soluble in 50 mg/ml 2 M NH4OH.
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