This product does not contain EDTA or other chelators.
C4482
CelLytic™ Y Cell Lysis Reagent
For yeast cells
Synonyma:
Yeast cell lysis reagent
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Vám/SKU | Dostupnost | Cena |
|---|---|---|
50 mL | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 2 580,00 Kč |
500 mL | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 14 500,00 Kč |
O této položce
NACRES:
NA.56
UNSPSC Code:
41116134
2 580,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohliGeneral description
CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.
Application
CelLytic™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4).
Features and Benefits
- Fast: Cell lysis in just 20 minutes
- Efficient: Three times more efficient than glass beads
- Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
- Gentle: Non-denaturing - preserves biological activity
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
1 of 1
Tato položka | |||
|---|---|---|---|
| description For yeast cells | description For bacterial cell lysis, 10× concentrate | description For bacterial cell lysis, 2× concentrate | description For bacterial cell lysis, standard strength |
| input bacteria and yeast sample(s) | input bacteria and yeast sample(s) | input bacteria and yeast sample(s) | input bacteria and yeast sample(s) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form liquid | form liquid | form solution |
Výstražné slovo
Danger
Kódy nebezpečí
Bezpečnostní pokyny
Skladovací třída
10 - Combustible liquids
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2
Seznamy omezení vyplývajících z předpisů
Seznamy omezení vyplývajících z předpisů jsou poskytovány zejména pro chemické produkty. Pro jiné než chemické produkty lze poskytovat pouze omezené informace. Žádný záznam znamená, že v seznamech není obsažena žádná ze součástí. Povinností uživatele je zajistit bezpečné a zákonné použití produktu.
9036-19-5
CAS No.
9036-19-5
CAS No.
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Does this contain EDTA or other chelators?
1 answer-
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Can I use CelLytic™ Y Cell Lysis Reagent, Product C4482, to make spheroplasts?
1 answer-
No. For spheroplast formation and protein extraction we recommend the CelLytic Y Plus kit.
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Should I use CelLytic™ Y Cell Lysis Reagent, Product C4482?
1 answer-
For all cases in which the addition of DTT does not interfere with the application tested, it is recommended to add DTT (5 - 10 mM final concentration) to the CelLytic Y reagent. Addition of DTT significantly improves total protein yield.
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What is in CelLytic™ Y Cell Lysis Reagent, Product C4482?
1 answer-
The formulation is considered proprietary, but we can state that it is a non-denaturing phosphate-free reagent.
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Can I use glass beads with CelLytic™ Y Cell Lysis Reagent, Product C4482?
1 answer-
If cell growth conditions are not optimal, then glass beads can be used to increase yields.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What strains have been used with CelLytic™ Y Cell Lysis Reagent, Product C4482?
1 answer-
The efficiency of CelLytic Y reagent for protein extraction has been tested on different strains of Saccharomyces cerevisiae [Y187, Y190, W303(a), S288C, SP1, S1278b (diploid and haploid), BJ2168, and cdc25] and Schizosaccharomyces pombe.
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