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D0547

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Synonyma:

DME/F-12, 1:1 mixture

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Vám/Skladová položkaDostupnostCena
10 x 1 L
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3 810,00 Kč

O této položce

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:

3 810,00 Kč


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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

  • to culture cerebral cortices from mice embryos
  • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
  • to culture human corneal epithelial cell lines

Preparation Note

Formulated to contain 12.0 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate

Other Notes

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

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Tato položka
D8900D2906D8062
description

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

description

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

description

With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

description

With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture

form

powder

form

powder

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

components

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

components

glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

400


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Questions

  1. Is this product supplied sterile, and does it arrive in sterile condition?

    1 answer
    1. This product is supplied as a powder cell culture medium and is not a sterile ready-to-use liquid product. Consequently, it is not sterile upon arrival. After reconstitution, the prepared medium must be sterile-filtered using a validated filtration method before use in sterile cell culture.

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