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D6546

DMEM - high glucose

Name: DMEM - high glucose
Brand: SIGMA
Price: 1260 CZK

With sodium Bicarbonate, sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Dulbecco’s Modified Eagle’s Medium - high glucose

1 of 2 reviewers received a sample product or took part in a promotion

Synonyma:

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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Vám/Skladová položka	Dostupnost	Cena
500 mL	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	1 260,00 Kč
6 x 500 mL	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	6 810,00 Kč

O této položce

UNSPSC Code:

12352207

NACRES:

NA.75

1 260,00 Kč

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Vlastnosti

Název produktu

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO₃: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

shipped in

ambient

storage temp.

2-8°C

Popis

General description

Listen to an Audio Overview
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Supplement with 0.584 g/L L-glutamine.

Other Notes

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Tato položka	D6429	D5796	D5671
Sigma-Aldrich D6546 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D6429 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D5796 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D5671 Dulbecco′s Modified Eagle′s Medium - high glucose
description With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture	description With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture	description With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma
form liquid	form liquid	form liquid	form liquid
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable
components NaHCO₃: yes phenol red: yes sodium pyruvate: yes HEPES: no L-glutamine: no glucose: high	components HEPES: no NaHCO₃: yes phenol red: yes sodium pyruvate: yes L-glutamine: yes glucose: high	components NaHCO₃: yes L-glutamine: yes glucose: high phenol red: yes sodium pyruvate: no HEPES: no	components L-glutamine: no glucose: high NaHCO₃: yes sodium pyruvate: no phenol red: yes HEPES: no
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Skladovací třída

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

Anonymous

2 years ago

Which DMEM media and glutamine products would you recommend for the PEA-13-97040311 cell line purchased from ECACC?

1 answer
1. Technical Support
  
  2 years ago
  
  According to ECACC, D6546 (DMEM) and G7513 (glutamine) are on the recommended product list for reagents required in cell culture applications.
  
  Helpful?

: 2 Reviews This action will navigate to reviews.