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D7295

Deoxynucleotide Mix, 10 mM

Molecular Biology Reagent

Synonyma:

Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs

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Vám/SKUDostupnostCena
0.2 mL
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
1 480,00 Kč
0.5 mL
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
2 850,00 Kč
5 mL
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19 700,00 Kč
20 x 0.2 mL
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28 300,00 Kč

O této položce

NACRES:
NA.52
UNSPSC Code:
41106305
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
liquid
Solubility:
H2O: soluble at 20 °C
Storage temp.:
−20°C

1 480,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
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biological source

synthetic (organic)

Quality Segment

assay

≥99%

form

liquid

concentration

10 mM

technique(s)

whole genome amplification: suitable

color

colorless

solubility

H2O: soluble at 20 °C

application(s)

agriculture
cannabis testing

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

General description

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

Application

dNTP Mix has been used:

  • in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
  • in reverse transcription of total RNA to cDNA.
  • as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
  • routine and long PCR
  • manual and automated DNA sequencing
  • cDNA synthesis and labeling reactions

Features and Benefits

  • Purity of each dNTP: Minimum 99%
  • Conveniently formulated; 1 μL is used per 50 μL PCR
  • Equimolar amounts of each dNTP means less pipetting
  • Minimize risk of contamination in PCR
  • UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

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Tato položka
DNTP100D6920NTMIXKB
Deoxynucleotide Mix, 10 mM Molecular Biology Reagent

Sigma-Aldrich

D7295

Deoxynucleotide Mix, 10 mM

Deoxynucleotide Set, 100 mM Individual dNTPs for routine PCR; 0.25 mL each

Sigma-Aldrich

DNTP100

Deoxynucleotide Set, 100 mM

2′-Deoxyadenosine 5′-triphosphate sodium salt solution 10 mM

Sigma-Aldrich

D6920

2′-Deoxyadenosine 5′-triphosphate sodium salt solution

KAPA dNTP Mix (10 mM each)

Roche

NTMIXKB

KAPA dNTP Mix (10 mM each)

description

Molecular Biology Reagent

description

Individual dNTPs for routine PCR; 0.25 mL each

description

10 mM

description

-

biological source

synthetic (organic)

biological source

-

biological source

-

biological source

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

-

form

liquid

form

liquid

form

liquid

form

liquid

assay

≥99%

assay

-

assay

≥99%

assay

>99% (HPLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Skladovací třída

12 - Non Combustible Liquids

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

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Osvědčení o analýze (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.

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Questions

1–5 of 5 Questions  

  1. Is this 10 mM each, or 10 mM total? This info should be clearly stated on the label and on your site.

    1 answer

    1. This is 10 mM each and can be found under Description-> General Description: The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. How much Product D7295, Deoxynucleotide Mix, is needed for a PCR reaction?

    1 answer

    1. One μl is sufficient for a standard 50 μl PCR reaction.

      Helpful?

  4. Does Product No. D7295, Deoxynucleotide Mix contain dTTP or TTP?

    1 answer

    1. The product contains Thymidine 5´-triphosphate sodium salt.  Thymidine has a deoxy sugar, so it is a deoxythymidine.

      Helpful?

  5. What are the components to Product D7295, Deoxynucleotide Mix?

    1 answer

    1. The D7295 contains 10mM of each dATP, dCTP, dGTP and TTP in molecular biology grade water.  This is for a total concentration of 40 mM.

      Helpful?

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