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D8417

4′,6-Diamidino-2-phenylindole dihydrochloride

powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence

Synonyma:

2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride

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Vybrat velikost

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Vám/SKUDostupnostCena
1 mg
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1 880,00 Kč
5 mg
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6 210,00 Kč
10 mg
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11 300,00 Kč

O této položce

Empirický vzorec (Hillův zápis):
C16H15N5 · 2HCl
Číslo CAS:
Molekulová hmotnost:
350.25
UNSPSC Code:
41116130
NACRES:
NA.75
PubChem Substance ID:
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:

1 880,00 Kč


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product line

BioReagent

Quality Segment

assay

≥98% (HPLC)

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 20 mg/mL, PBS: insoluble

ε (extinction coefficient)

30 at 263 nm in H2O

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

General description

Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.
4′,6-Diamidino-2-phenylindole dihydrochloride is a fluorescent dye for staining DNA. It strongly interacts with the dAT-rich (deoxy adenine-thymine) regions of the DNA.

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
4′,6-Diamidino-2-phenylindole dihydrochloride has been used in staining of various cells in immunocytochemistry and cytochemistry, human subcutaneous adipose tissues for fluorescence imaging, embryo sections for immunofluorescence.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Disclaimer

Protect from light.

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Tato položka
32670MBD0015MBD0020
description

powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence

description

suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

description

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

description

For Nuclear counterstain in Fluorescence Microscopy

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for nucleic acid staining

suitability

suitable for nucleic acid staining

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

transfection: suitable

technique(s)

DNA sequencing: suitable, PCR: suitable, DNA extraction: suitable

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI)

form

powder

form

powder

form

liquid

form

liquid

solubility

H2O: 20 mg/mL, PBS: insoluble

solubility

DMF: soluble, H2O: soluble

solubility

water: soluble

solubility

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200


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Piktogramy

Exclamation mark

Výstražné slovo

Warning

Kódy nebezpečí

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

Cílové orgány

Respiratory system

Skladovací třída

11 - Combustible Solids

WGK

WGK 3

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

PPE (osobní ochranné prostředky)

Eyeshields, Gloves, type N95 (US)



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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