F4274

Fluorescein isothiocyanate isomer I

≥97.5% purity (HPLC), powder

• Synonyma: FITC, Fluorescein 5-isothiocyanate
• Empirický vzorec (Hillův zápis): C₂₁H₁₁NO₅S
• Číslo CAS: 3326-32-7
• Molekulová hmotnost: 389.38
• NACRES: NA.47
• PubChem Substance ID: 24894860
• UNSPSC Code: 12171500
• EC Number: 222-042-0
• MDL number: MFCD00005063
• Beilstein/REAXYS Number: 1407295

Vlastnosti

• Název produktu: Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
• assay: ≥97.5% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow to very dark yellow
• mp: >360 °C (lit.)
• solubility: acetone: soluble 10 mg/mL
• fluorescence: λ_ex 492 nm; λ_em 518 nm (green)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Popis

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Bezpečnostní informace

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves