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G7765

Gelatin from cold water fish skin

40-50% in H2O

Synonyma:

Gelatine, Teleostean gelatin

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Vám/SKUDostupnostCena
1 L
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10 500,00 Kč

O této položce

Číslo CAS:
MDL number:
NACRES:
NA.61
UNSPSC Code:
12352202

10 500,00 Kč


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biological source

fish skin

Quality Segment

form

liquid

mol wt

~60 kDa

contains

0.15% propyl p-hydroxybenzoate as preservative, 0.2% methyl p-hydroxybenzoate as preservative

concentration

40-50% in H2O

technique(s)

ELISA: suitable, cell culture | mammalian: suitable, immunocytochemistry: suitable, western blot: suitable

storage temp.

2-8°C

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water.
Gelatin is derived from collagen and is the most abundant protein in the extracellular matrix (ECM) of the tissues. It is a bioactive material. Gelatin consists of matrix metalloproteinase (MMP) sensitive protein sequences.

Application

Gelatin from cold water fish skin has been used in an incubation medium (IM) in immunolabelling of rat brain sections, as a component of rinse buffer in western blotting of various cells, as a blocking agent in immunofluorescent staining of HeLa cells.
Used as a blocking agent in immunochemistry.

Biochem/physiol Actions

Gelatin is cross-linked either chemically or enzymatically to form a three dimensional (3D) hydrogel.

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Tato položka
G1890G2625G0411
description

40-50% in H2O

description

powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

description

gel strength ~175 g Bloom, Type A

description

aqueous solution

technique(s)

ELISA: suitable, cell culture | mammalian: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

western blot: suitable, microbiological culture: suitable, ELISA: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

powder

form

powder

form

aqueous solution

storage temp.

2-8°C

storage temp.

room temp

storage temp.

-

storage temp.

-

contains

0.15% propyl p-hydroxybenzoate as preservative, 0.2% methyl p-hydroxybenzoate as preservative

contains

-

contains

-

contains

-

biological source

fish skin

biological source

Porcine skin

biological source

Porcine skin

biological source

Porcine bone


Skladovací třída

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–6 of 6 Questions  
  1. What is the molecular weight of this Gelatin?

    What is the molecular weight of this Gelatin?

    1 answer
    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

  2. What is the difference between type A and type B gelatin?

    1 answer
    1. Type A gelatin is derived from acid-cured tissue whereas Type B gelatin is derived from lime-cured tissue. Type A gelatin has about 78-80 millimoles of free carboxyl groups per 100 g of protein and a pI of 7.9-9.0. Type B has 100-115 millimoles of free carboxyl groups per 100 g of protein and a pI of 4.7-5.2.

      Helpful?

  3. What is the solution stability of Product G7765, Gelatin from cold water fish skin?

    1 answer
    1. The specification for shelf life is 2 years for unopened bottles stored at 2-8°C.  Each lot is assigned a retest date; this date is given on the Certificate of Analysis avialable from our website.

      Helpful?

  4. What is the gel point of Product G7765, Gelatin from cold water fish skin?

    1 answer
    1. The gel point of Product G7765, Gelatin from cold water fish skin is 5-10°C. The product will be a gel when storing the product at 2-8°C.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is Product G7765, Gelatin from cold water fish skin, soluble in?

    1 answer
    1. Gelatin is soluble in glycerol and acetic acid, and more soluble in hot water than in cold water.

      Helpful?

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