H5527

Hygromycin B solution from Streptomyces hygroscopicus

≥60% (HPLC), 45-60 mg/mL in H₂O, γ-irradiated

• Synonyma: Hygromycin b, Hygrovetine
• Empirický vzorec (Hillův zápis): C₂₀H₃₇N₃O₁₃
• Číslo CAS: 31282-04-9
• Molekulová hmotnost: 527.52
• UNSPSC Code: 51102829
• NACRES: NA.85
• PubChem Substance ID: 24895698
• MDL number: MFCD06795479
• Beilstein/REAXYS Number: 6755837

Vlastnosti

• biological source: Streptomyces hygroscopicus
• Quality Segment: 200
• sterility: γ-irradiated
• concentration: ≥60% (HPLC), 45-60 mg/mL in H₂O
• color: yellow-brown to orange
• antibiotic activity spectrum: viruses
• mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChI key: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Popis

General description

Chemical structure: aminoglycoside

Application

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Biochem/physiol Actions

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Preparation Note

This product is a solution that is =60% (HPAE), 45-60 mg/mL in H₂O, and γ-irradiated .

Disclaimer

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Bezpečnostní informace

• Piktogramy: GHS06,GHS08,GHS05
• Výstražné slovo: Danger
• Kódy nebezpečí: H301 + H311,H318,H330,H334
• Bezpečnostní pokyny: P260 - P280 - P284 - P301 + P310 - P305 + P351 + P338 - P310
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Resp. Sens. 1
• Skladovací třída: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Co se děje?: WGK 3
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter