The image below is stained with Brilliant Blue R-250
L4631
Lysozyme from chicken egg white
For use as a marker in SDS-PAGE
Synonyma:
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
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| Vám/SKU | Dostupnost | Cena |
|---|---|---|
1 vial | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 6 360,00 Kč |
O této položce
Číslo CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
235-747-3
MDL number:
Číslo EC:
Biological source:
chicken egg white
6 360,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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chicken egg white
Quality Segment
form
powder
mol wt
single-chain 14.3 kDa
packaging
vial of 25 mg
input
bacteria and yeast sample(s)
UniProt accession no.
storage temp.
2-8°C
Gene Information
chicken ... LYZ(396218)
Application
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
Other Notes
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
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Tato položka | |||
|---|---|---|---|
| description For use as a marker in SDS-PAGE | description dialyzed, lyophilized, powder, ~100000 U/mg | description powder or granules, ≥39,000 units/mg protein | description Grade VI, ≥35,000 units/mg protein (E1%/282) |
| Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information - |
| biological source chicken egg white | biological source chicken egg white | biological source chicken egg white | biological source chicken egg white |
| form powder | form powder | form powder or granules | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. room temp | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. - |
| mol wt single-chain 14.3 kDa | mol wt single-chain 14.3 kDa, Mr ~14600 | mol wt single-chain 14.3 kDa | mol wt ~14.3 kDa |
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Výstražné slovo
Danger
Kódy nebezpečí
Bezpečnostní pokyny
Hazard Classifications
Resp. Sens. 1
Skladovací třída
11 - Combustible Solids
Co se děje?
WGK 3
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
PPE (osobní ochranné prostředky)
Eyeshields, Gloves, type N95 (US)
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