NA0600

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit

1 kit sufficient for 5 preparations

• Synonyma: Gen Elute, GenElute Endotoxin-Free Plasmid Kit
• NACRES: NA.52
• UNSPSC Code: 41105501

Vlastnosti

• Quality Segment: 200
• usage: kit sufficient for 5 preparations
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• test parameters: sample volume: 200-1000 mL bacterial culture
• greener alternative category: , Aligned
• storage temp.: 15-25°C

Popis

General description

The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with ≤ 0.1 endotoxin units/μg can be recovered from 200 mL - 1.0 L LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digest, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Click here to view the kit protocol

Application

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

Features and Benefits

• Up to 5 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
• Only 90 minutes from pelleted cells to purified plasmid
• Vacuum format with no ethanol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Součásti soupravy, které jsou dostupné také samostatně

• G3546

  GenElute^™ HP Pro Megaprep Binding Columns 5

  Bezpečností list
• C2112

  Column Preparation Solution 2

  Bezpečností list
• N7411

  Neutralization Solution EF

  Bezpečností list
• L1912

  Lysis Solution

  Bezpečností list
• 2107

  E-Toxate^™ Water, endotoxin, free

  Bezpečností list
• W4639

  Wash Solution 2

  Bezpečností list
• R1149

  Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )

  Bezpečností list

Zobrazit všechny (11)

Bezpečnostní informace

• Piktogramy: GHS02,GHS08,GHS05,GHS07
• Výstražné slovo: Danger
• Kódy nebezpečí: H226,H290,H302 + H332,H315,H319,H334,H336,H372
• Bezpečnostní pokyny: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
• Cílové orgány: Central nervous system, Lungs
• Skladovací třída: 3 - Flammable liquids
• Bod vzplanutí (°F): 77.0 °F - closed cup
• Bod vzplanutí (°C): 25 °C - closed cup
• Co se děje?: WGK 3