Poly-D-lysine hydrobromide with a molecular weight greater than 30,000 Da is recommended for cell attachment. The poly-D-lysine hydrobromide with a molecular weight of 84,000 Da has more attachment sites per polymer chain available to the cells. This type is most suitable for cell attachment.
P1024
Poly-D-lysine hydrobromide
suitable for cell culture, Mol wt ≥300,000
Synonyma:
D-Lysine homopolymer hydrobromide
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| Vám/SKU | Dostupnost | Cena |
|---|---|---|
10 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 1 560,00 Kč |
50 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 4 600,00 Kč |
100 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 7 840,00 Kč |
500 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 25 700,00 Kč |
1 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 38 800,00 Kč |
O této položce
Lineární vzorec:
D-Lys-(D-Lys)n-D-Lys · xHBr
Číslo CAS:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.26
MDL number:
eCl@ss:
32150312
1 560,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohliNázev produktu
Poly-D-lysine hydrobromide, mol wt ≥300,000
form
powder or solid
Quality Segment
mol wt
≥300,000
technique(s)
cell culture | mammalian: suitable
color
white to off-white
storage temp.
−20°C
SMILES string
O=C(C)[C@@](NC)([H])CCCCN.[Br]
InChI
1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
InChI key
MEXAGTSTSPYCEP-UHFFFAOYSA-N
Application
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.
This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
Biochem/physiol Actions
Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.
Preparation Note
This product has a molecular weight >300,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.
Other Notes
Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the β structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
Disclaimer
Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.
1 of 1
Tato položka | |||
|---|---|---|---|
| description mol wt ≥300,000 | description mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture | description mol wt 30,000-70,000 | description average mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture |
| form powder or solid | form lyophilized powder | form solid | form lyophilized powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| color white to off-white | color - | color white to off-white | color - |
| mol wt ≥300,000 | mol wt 70,000-150,000 | mol wt 30,000-70,000 | mol wt average mol wt 30,000-70,000 |
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Skladovací třída
11 - Combustible Solids
WGK
WGK 3
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
PPE (osobní ochranné prostředky)
Eyeshields, Gloves, type N95 (US)
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