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P6774

Phosphatase, Alkaline from bovine intestinal mucosa

Name: Phosphatase, Alkaline from bovine intestinal mucosa
Brand: SIGMA
Price: 2250 CZK

buffered aqueous solution, ≥2,000 DEA units/mg protein

Akční nabídka

Synonyma:

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Vám/SKU	Dostupnost	Cena
1000 units	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	2 250,00 Kč
2000 units	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	3 340,00 Kč
10000 units	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	11 700,00 Kč
50000 units	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	45 200,00 Kč

O této položce

Číslo CAS:

9001-78-9

UNSPSC Code:

12352204

eCl@ss:

42010105

NACRES:

NA.54

Číslo EC:

3.1.3.1 (BRENDA, IUBMB)

MDL number:

MFCD00131849

Specific activity:

≥2,000 DEA units/mg protein

Biological source:

bovine intestinal mucosa

Concentration:

5-20 mg/mL

2 250,00 Kč

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Vlastnosti

biological source

bovine intestinal mucosa

Quality Segment

200

form

buffered aqueous solution

specific activity

≥2,000 DEA units/mg protein

mol wt

dimer ~160 kDa

concentration

5-20 mg/mL

storage temp.

2-8°C

Popis

General description

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins, such as casein, and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. Product P6774 has been used during preparation of brain lysate.

The enzyme from Sigma has been used in the preparation of AP-BGG (alkaline phosphatase-bovine gamma globulin) conjugates. It has been conjugated to sheep anti-rabbit IgG and sheep anti-rabbit IgM in immunological assays. It has also been used to prepare double-enzyme conjugates with HRP for the simultaneous detection of three different intracellular Ig determinants in a single tissue section.

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

The enzyme is a glycoprotein containing approximately 12% carbohydrate (6% hexoses and 6% other neutral sugars). Each molecule of alkaline phosphatase contains four zinc atoms and four disulfide bridges. Maximal activity with alkaline phosphatase is achieved in the presence of magnesium. It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate, phenyl phosphate, phenolphthalein phosphate, α-glycerol phosphate, β-glycerol phosphate, 2-phosphorylglycerate, triosephosphate, glucose-6-phosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, adenosine 5-phosphate adenosine 3-phosphate, phosphoenolpyruvate, and β-nicotinamide adenine dinucleotide phosphate. Arsenate, cysteine, iodine, inorganic phosphate, pyrophosphate, diisopropyl phosphate, triphenylphosphate, diisopropyl fluorophosphate, and L-phenylalanine are some of the strong inhibitors of alkaline phosphatase.

Alkaline phosphatase, from bovine intestinal mucosa, is most stable in the pH range 7.5-9.5. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols and it requires zinc, and magnesium or calcium divalent ions for activity.

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical form

Solution in 3.0 M NaCl containing 5 mM MgCl₂, 0.2 mM ZnCl₂, and 30 mM triethanolamine, pH 7.6

Preparation Note

Affinity purified

Analysis Note

Package sizes are based on DEA units

Protein determined by biuret.

Other Notes

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

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Tato položka	P4069	P5931	SAE0111
Sigma-Aldrich P6774 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P4069 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich P5931 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich SAE0111 Phosphatase, Alkaline from human placenta
description buffered aqueous solution, ≥2,000 DEA units/mg protein	description buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)	description lyophilized powder, 30-60 units/mg protein (in glycine buffer)	description ≥10 units/mg solid (in glycine buffer)
specific activity ≥2,000 DEA units/mg protein	specific activity 20-50 units/mg protein (in glycine buffer)	specific activity 30-60 units/mg protein (in glycine buffer)	specific activity ≥10 units/mg solid (in glycine buffer)
biological source bovine intestinal mucosa	biological source Escherichia coli	biological source -	biological source human placenta
form buffered aqueous solution	form buffered aqueous glycerol solution	form lyophilized powder	form -
concentration 5-20 mg/mL	concentration -	concentration -	concentration -
mol wt dimer ~160 kDa	mol wt -	mol wt -	mol wt -
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C