The M3168 product does not require a stop solution. 4MUP is a fluorescent substrate. Adding a stop solution to 4MUP, such as an acidic solution like 2M Sulfuric Acid, would greatly reduce or eliminate the fluorescence. While stop solutions are typically added to substrates like pNPP, TMB, or OPD, they are not required when working with product M3168. The Product Information Sheet for M3168 can be downloaded from the website, where it mentions that the solution is ready to use and nothing else should be added to it.
M3168
4-Methylumbelliferyl phosphate
alkaline phosphatase substrate, liquid
Synonyma:
4-methylumbelliferone phosphate Liquid Substrate System
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Vybrat velikost
Změnit zobrazení
| Vám/SKU | Dostupnost | Cena |
|---|---|---|
100 mL | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 6 930,00 Kč |
O této položce
Číslo CAS:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32
EC Number:
222-137-7
MDL number:
6 930,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohliNázev produktu
4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, liquid
form
liquid
Quality Segment
storage temp.
2-8°C
SMILES string
CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12
InChI
1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
InChI key
BCHIXGBGRHLSBE-UHFFFAOYSA-N
General description
4-Methylumbelliferyl phosphate (4-MUP) is a substrate for alkaline phosphatase which forms the soluble fluorescent substance methylumbelliferone. 4-MUP Liquid Substrate System combines 4-MUP and buffer in a ready-to-use reagent.
Application
4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System has been used as a fluorescent substrate for alkaline phosphate in enzyme linked immunosorbent assay (ELISA). It has also been used in secreted embryonic alkaline phosphatase (SEAP) activity assay.
Analysis Note
4-MUP fluorescence can be measured with excitation at 360 nm and emission at 440 nm. The fluorescent end product may also be observed using a UV light source.
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Tato položka | |||
|---|---|---|---|
| description liquid | description phosphatase substrate | description ready to use solution | description ready to use solution |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form liquid | form powder | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
Skladovací třída
11 - Combustible Solids
Co se děje?
WGK 3
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
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Which stop solution is the best option for AP-4MUP ELISA? Please advise.
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