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R0883

RPMI-1640 Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonyma:

Roswell Park Memorial Institute 1640 medium

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Vám/SKUDostupnostCena
100 mL
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411,00 Kč
500 mL
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1 090,00 Kč
1 L
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1 510,00 Kč
6 x 500 mL
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6 160,00 Kč
6 x 1 L
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8 180,00 Kč
24 x 500 mL
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19 400,00 Kč

O této položce

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:

411,00 Kč


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Název produktu

RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used:
  • to culture PC-3 prostate cell line and Jurkat cells
  • to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line
  • to cultivate circulating tumor cells (CTCs)

Preparation Note

Supplement with 0.3 g/L L-glutamine.

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RPMI-1640 Medium

description

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

AutoMod™, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture

description

HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture

description

Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

powder

form

liquid

form

liquid

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

components

sodium pyruvate: no
HEPES: no
NaHCO3: no
L-glutamine: no
phenol red: yes

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes

components

phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C


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Skladovací třída

12 - Non Combustible Liquids

Co se děje?

WGK 2

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

  1. Could you tell me the exact concentration of glucose in this RPMI-1640 medium(cat#R0883 ), please ?

    1 answer
    1. For this RPMI formulation, the glucose concentration is 2 grams per liter.

      Please see the datasheet below which includes the formulations of all classical RPMI-1640 media products:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/229/770/r0883for.pdf

      Helpful?

Reviews

Active Filters

  1. Boston, MA
    • Reviews 3
    • Votes 14
    5 out of 5 stars.

    Great product!

    Reliable cell culture product that gets frequent use

    Helpful?