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R6148

RNase A solution

Synonyma:

GenElute™ RNAse A

Přihlásit pro zobrazení organizačních a smluvních cen.

Vybrat velikost

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Vám/SKUDostupnostCena
25 mL
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8 930,00 Kč

O této položce

NACRES:
NA.52
UNSPSC Code:
12352204
Specific activity:
1500 units/mL, 3000 units/mL
Biological source:
bovine pancreas
Concentration:
20 mg/mL in Tris buffer

8 930,00 Kč

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biological source

bovine pancreas

Quality Segment

form

liquid

specific activity

1500 units/mL, 3000 units/mL

concentration

20 mg/mL in Tris buffer

technique(s)

DNA purification: suitable

storage temp.

room temp

Gene Information

General description

Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute™ kits.

Application

RNase A solution has been used:

  • in apoptosis assay of Hep-2 cells
  • for the colorimetric quantification of methylated DNA
  • for DNA and RNA extraction
  • in DNA laddering analysis
  • in cell cycle analysis
  • as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry

Biochem/physiol Actions

Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.

Preparation Note

For usage requirements, please refer to the kit procedure.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Tato položka
70856E1656B9560
RNase A solution

Sigma-Aldrich

R6148

RNase A solution

RNase A Solution Convenient solution for selective degradation of RNA

Millipore

70856

RNase A Solution

Endocleaning Solution

Sigma-Aldrich

E1656

Endocleaning Solution

Binding Solution

Sigma-Aldrich

B9560

Binding Solution

description

-

description

Convenient solution for selective degradation of RNA

description

-

description

-

technique(s)

DNA purification: suitable

technique(s)

DNA purification: suitable

technique(s)

-

technique(s)

-

specific activity

3000 units/mL, 1500 units/mL

specific activity

-

specific activity

-

specific activity

-

Gene Information

bovine ... RNASE1(282340), RNASE1(280930)

Gene Information

-

Gene Information

-

Gene Information

-

biological source

bovine pancreas

biological source

-

biological source

-

biological source

-

form

liquid

form

solution

form

-

form

liquid

concentration

20 mg/mL in Tris buffer

concentration

10 mg/mL

concentration

-

concentration

-

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Piktogramy

Health hazard
GHS08

Výstražné slovo

Danger

Kódy nebezpečí

H334

Bezpečnostní pokyny

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Skladovací třída

10 - Combustible liquids

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  

  1. Si può congelare in aliquote? O tenere aliquote a 4 C? Per quanto tempo l'attività si mantiene invariata se conservata a temp. ambiente? In quali buffers si può diluire durante l'uso?

    1 answer

    1. This solution is quite stable and not assigned and expiration or retest date. The product is 20 mg/mL ribonuclease A solution prepared in Tris buffer with 50% glycerol. Further dilutions may be made using a Tris or Tris-EDTA. This item may be stored at room temperature. Stability studies at refrigerated or freezer temperatures have not been conducted and may not extend the shelf-life of the product. Products that are not assigned an expiration or retest date are warranted for one year from the date of shipment.

      Helpful?

  2. Qual a temperatura ótima da enzima? E qual a temperatura de armazenamento?

    1 answer

    1. This product can be stored at room temperature due to its stability. Optimal temperature information is not available for this product; however, a similar product indicates that the optimal temperature is 60 °C.

      Helpful?

  3. what is the total volume of this product?

    1 answer

    1. The total volume in this item is 25 mL.

      Helpful?

  4. What is the concentration of the RNase a solution in product R6148?

    1 answer

    1. R6148 is solution of 20 mg/ml RNAse A in Tris Buffer with 50% glycerol. A rough estimate of activity is 1000-2000 Kunitz units/ml.

      Helpful?

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