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R8755

RPMI-1640 Medium

With ʟ-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

Synonyma:

Roswell Park Memorial Institute 1640 medium

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Vám/SKUDostupnostCena
10 x 1 L
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2 380,00 Kč
10 L
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2 540,00 Kč

O této položce

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217820

2 380,00 Kč

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Název produktu

RPMI-1640 Medium, Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

Listen to an Audio Overview
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium is used for the following applications:
  • Used in cell culture medium: Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components
  • Used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
  • Used for cell isolation and culture
  • Used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo Proliferation Assay
  • Used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
  • Used for the preparation of antifungal agents

Preparation Note

Formulated to contain 10.4 grams of powder per liter of medium.
Supplement with 2.0 g/L sodium bicarbonate.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use.

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Tato položka
R1383R1145R8758
RPMI-1640 Medium Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

R8755

RPMI-1640 Medium

RPMI-1640 Medium With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

R1383

RPMI-1640 Medium

RPMI-1640 Medium 10 ×, Without L-glutamine, folic acid and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

SAFC

R1145

RPMI-1640 Medium

RPMI-1640 Medium With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R8758

RPMI-1640 Medium

description

Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

description

With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

description

10 ×, Without L-glutamine, folic acid and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

description

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

form

powder

form

powder

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no

components

L-glutamine: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells

classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells

classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells

RPMI, RPMI 1640, RPMI Medium 1640, lymphoblastoid, dendritic, hematopoietic, differentiation, AML, myleloid, myelodysplastic, CMB, myeloma, KM3, 1640 medium, RPMI medium, RPMI, RPMI1640

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Skladovací třída

11 - Combustible Solids

Co se děje?

WGK 2

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

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Osvědčení o analýze (COA)

Lot/Batch Number

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