S9012

Sulforhodamine B sodium salt

Powder

• Synonyma: Acid red 52; Acid Rhodamine B
• Empirický vzorec (Hillův zápis): C₂₇H₂₉N₂NaO₇S₂
• Číslo CAS: 3520-42-1
• Molekulová hmotnost: 580.65
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24899824
• EC Number: 222-529-8
• MDL number: MFCD00010180
• Colour Index Number: 45100
• Beilstein/REAXYS Number: 3886008

Vlastnosti

• Název produktu: Sulforhodamine B sodium salt, Technical grade
• Quality Segment: 200
• form: powder
• composition: Dye content, ~75%
• εmax: ≥85000 at 554-560 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1
• InChI: 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
• InChI key: SXQCTESRRZBPHJ-UHFFFAOYSA-M

Popis

General description

Sulforhodamine B (also known as SRB, kiton rhodamine B, lissamine rhodamine B, or sulphorhodamine B) is a bright-pink anionic, aminoxanthene dye carrying two sulfonate substituents.

Application

Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.

It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.

It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)