SEQXE
SeqPlex DNA Amplification Kit
For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.
Synonyma:
SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit
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Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 10 reactions | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 8 820,00 Kč | |
| 50 reactions | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 37 600,00 Kč | |
| 500 reactions | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 271 200,00 Kč |
O této položce
NACRES:
NA.55
UNSPSC Code:
41121800
8 820,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohliQuality Level
technique(s)
whole genome amplification: suitable
dilution
(WGA)
input
purified DNA
shipped in
wet ice
storage temp.
−20°C
General description
SeqPlex™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD™, or 454 sequencing workflows.
Application
SeqPlex™ DNA Amplification Kit has been used for whole genome amplification.
Features and Benefits
- Random priming technology amplifies fragmented DNA such as ChIP or FFPE
- Facilitates sequencing from as little as 100 pg of ChIP DNA
- Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
- Compatible with Illumina®, SOLiD™, or 454 library prep for next generation sequencing
Other Notes
SEQXE-500RXN is manufactured on-demand. Contact technical services at [email protected] for more information.
Legal Information
Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
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Tato položka | |||
|---|---|---|---|
| technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable |
| input purified DNA | input purified RNA | input purified DNA | input purified DNA |
| dilution (WGA) | dilution (WTA) | dilution (WGA) | dilution (WGA) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Součásti soupravy, které jsou dostupné také samostatně
Č. produktu
Popis
Bezpečností list & Stanovení ceny
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Skladovací třída
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Protokoly
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Související obsah
Instructions
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Takuya Hayakawa et al.
Cytogenetic and genome research, 161(8-9), 437-444 (2021-11-25)
E/L Repli-seq is a powerful tool for detecting cell type-specific replication landscapes in mammalian cells, but its potential to monitor DNA replication under replication stress awaits better understanding. Here, we used E/L Repli-seq to examine the temporal order of DNA
Joshua A Udall et al.
Frontiers in plant science, 10, 1541-1541 (2019-12-13)
One of the extraordinary aspects of plant genome evolution is variation in chromosome number, particularly that among closely related species. This is exemplified by the cotton genus (Gossypium) and its relatives, where most species and genera have a base chromosome
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| SEQXE-10RXN | 04061837018138 |
| SEQXE-50RXN | 04061837018176 |
| SEQXE-500RXN | 04061837018145 |