SHC005

MISSION^® pLKO.1-puro eGFP shRNA Control Plasmid DNA

shRNA sequence targeting eGFP

• Synonyma: MISSION^® Control Vectors
• UNSPSC Code: 41106609
• NACRES: NA.51
• MDL number: MFCD07785395
• Concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Vlastnosti

• Quality Segment: 200
• product line: MISSION^®
• concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
• shipped in: dry ice
• storage temp.: −20°C

Popis

General description

The MISSION^® eGFP shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761). The eGFP shRNA Control Vector is useful as a positive knockdown control in experiments using cell lines expressing eGFP.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids, MISSION^® Lentiviral Packaging Mix (Prod. No. SHP001). The eGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• Co se děje?: WGK 1
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable