T3913

Tris-Borate-EDTA buffer

5× concentrate, powder blend

• Synonyma: TBE buffer
• NACRES: NA.25
• PubChem Substance ID: 329826779
• UNSPSC Code: 41105319
• MDL number: MFCD00236358

Vlastnosti

• Quality Segment: 200
• form: powder blend
• technique(s): electrophoresis: suitable
• impurities: DNase, RNase and NICKase, none detected
• pH: 8.1-8.5 (25 °C, 5 ×)
• solubility: water: 85.1 g/L, clear, colorless
• suitability: suitable for gel electrophoresis (after dilution to working concentration)
• application(s): diagnostic assay manufacturing
• SMILES string: OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
• InChI key: OSBLTNPMIGYQGY-UHFFFAOYSA-N

Popis

Application

Tris-Borate-EDTA buffer is suitable for use in gel electrophoresis.
It may be employed for the following studies:

• As buffer system for the evaluation of molecular weights of glycoproteins of ovalbumin, avidin and fetuin by sodium dodecyl sulfate-pore gradient electrophoresis.
• For the hemoglobin electrophoresis in starch gel.
• Preparation of buffer concentration gradient gel for the rapid determination of DNA sequence.

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

Packaged in pouches

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. A suitable container must be supplied.

Bezpečnostní informace

• Piktogramy: GHS08
• Výstražné slovo: Danger
• Kódy nebezpečí: H360FD
• Bezpečnostní pokyny: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Skladovací třída: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Co se děje?: WGK 1
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type P2 (EN 143) respirator cartridges