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T6025

Tris Acetate-EDTA buffer

BioReagent, suitable for electrophoresis

Synonyma:

TAE buffer

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Vám/SKUDostupnostCena
1 L
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1 990,00 Kč

O této položce

NACRES:
NA.25
PubChem Substance ID:
329826837
UNSPSC Code:
41105319
MDL number:
MFCD00236357

1 990,00 Kč

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sterility

0.2 μm filtered

Quality Segment

product line

BioReagent

form

working solution

technique(s)

electrophoresis: suitable

impurities

DNase, RNase, Protease, none detected

suitability

suitable for electrophoresis, suitable for gel electrophoresis (after dilution to working concentration)

application(s)

diagnostic assay manufacturing

SMILES string

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

HGEVZDLYZYVYHD-UHFFFAOYSA-N

Application

Tris Acetate-EDTA buffer has been used as run buffer for the agarose gel electrophoresis of human papillomavirus DNA and transcribed RNA samples.
TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Solution prepared with 18 megohm water

Other Notes

40 mM Tris acetate, pH approx. 8.3, containing 1 mM EDTA.

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Tato položka
T3913T7527T4415
Tris Acetate-EDTA buffer BioReagent, suitable for electrophoresis

Sigma-Aldrich

T6025

Tris Acetate-EDTA buffer

Tris-Borate-EDTA buffer 5× concentrate, powder blend

Sigma-Aldrich

T3913

Tris-Borate-EDTA buffer

Tris-Borate-EDTA buffer BioReagent, Molecular Biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Sigma-Aldrich

T7527

Tris-Borate-EDTA buffer

Tris-Borate-EDTA buffer BioReagent, suitable for electrophoresis, 10× concentrate

Sigma-Aldrich

T4415

Tris-Borate-EDTA buffer

description

BioReagent, suitable for electrophoresis

description

5× concentrate, powder blend

description

BioReagent, Molecular Biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

description

BioReagent, suitable for electrophoresis, 10× concentrate

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

product line

BioReagent

product line

-

product line

BioReagent

product line

BioReagent

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

form

working solution

form

powder blend

form

powder blend

form

solution

impurities

DNase, RNase, Protease, none detected

impurities

DNase, RNase and NICKase, none detected

impurities

DNase and RNase, none detected

impurities

DNase and RNase, none detected

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Skladovací třída

12 - Non Combustible Liquids

Co se děje?

WGK 2

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

PPE (osobní ochranné prostředky)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Could you provide the concentration of product T6025 and clarify if it is considered 50X, 10X, or 1X?

    1 answer

    1. The concentration for product T6025 is 40 mM Tris acetate with 1 mM EDTA at approximately pH 8.3. This solution, prepared with 18 megohm water and 0.2 μm filtration, is considered a 1X working solution.

      Helpful?

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