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T8782

Tryptose Phosphate Broth

buffered powder, Microbiologically tested.

Synonyma:

Tryptose Broth

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Vám/SKUDostupnostCena
1 kg
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5 160,00 Kč
500 g
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5 570,00 Kč
10 kg
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44 900,00 Kč

O této položce

NACRES:
NA.75
UNSPSC Code:
41106507

5 160,00 Kč


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form

buffered powder

Quality Segment

quality

Microbiologically tested.

General description

The tryptose component of Tryptose Phosphate Broth is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein.

Application

In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Tryptose Phosphate Broth has been used as a component:
  • of Leibovitz L-15 medium for the culture of BME26 tick embryo cell line
  • of M199 medium for the preparation of chick embryo fibroblasts (CEFs)
  • of Glasgow′s minimum essential medium (GMEM) for culturing baby hamster kidney (BHK-21) before transfection

Biochem/physiol Actions

Tryptose Phosphate Broth provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious micro-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

Other Notes

Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

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Tato položka
T8159T4407T9157
description

buffered powder, Microbiologically tested.

description

sterile-filtered, suitable for cell culture

description

suitable for microbiology, NutriSelect® Plus

description

suitable for insect cell culture

form

buffered powder

form

solution

form

powder

form

solid

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

quality

Microbiologically tested.

quality

-

quality

-

quality

-


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Skladovací třída

11 - Combustible Solids

Co se děje?

WGK 1

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

PPE (osobní ochranné prostředky)

Eyeshields, Gloves, type N95 (US)



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