The concentration of dye in 10,000X GelRed is approximately 10 mM.
SCT123
GelRed® Nucleic Acid Stain 10000X Water
GelRed is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.
Synonym(s):
Fluorescent DNA gel stain
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form
liquid
technique(s)
PCR: suitable
solubility
water: soluble
suitability
suitable for nucleic acid staining
detection method
fluorometric
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This Item | SCT122 | SCT125 | SCT124 |
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) PCR: suitable | technique(s) PCR: suitable | technique(s) PCR: suitable |
| suitability suitable for nucleic acid staining | suitability - | suitability suitable for nucleic acid staining | suitability suitable for nucleic acid staining |
| form liquid | form - | form liquid | form liquid |
| solubility water: soluble | solubility - | solubility water: soluble | solubility water: miscible |
microbialanalysis | microbialanalysis | - | microbialanalysis |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
General description
GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed and EtBr have virtually the same spectra (Figure 1), so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed is far more sensitive than EtBr (Figure 2).
The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelRed successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelRed is not classified as hazardous waste.
Spectral Properties
Absorbance: Standard Transilluminator (302 or 312 nm)
Emission: Ethidium Bromide, SYBR or GelStar Filter
The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelRed successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelRed is not classified as hazardous waste.
Spectral Properties
Absorbance: Standard Transilluminator (302 or 312 nm)
Emission: Ethidium Bromide, SYBR or GelStar Filter
Application
GelRed is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.
Nucleic acid detection
Research Category
Cell Culture
Cell Culture
Research Sub Category
Live Cell Dye
Live Cell Dye
Physical form
Liquid
Preparation Note
GelRed is a very stable dye. Store GelRed at room temperature, protected from light. Dye precipitation may occur at lower temperatures, resulting in lower signal or the appearance of precipitate on the surface of the gel. If this occurs, heat the solution to 45-50°C for two minutes and vortex. Protect From Light.
Analysis Note
Spectral Properties
Absorbance: Standard Transilluminator (302 or 312 nm)
Emission: Ethidium Bromide, SYBR or GelStar Filter
Absorbance: Standard Transilluminator (302 or 312 nm)
Emission: Ethidium Bromide, SYBR or GelStar Filter
Legal Information
GelRed is a registered trademark of Biotium Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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I would like to use GelRed to visualize DNA-protein binding but the dye cannot be in excess of DNA. Therefore, for accurate calculations, what is the molarity of the 10000x stock solution?
1 answer-
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Hello, I want to order this DNA dye (GelRed® Nucleic Acid Stain 10000X Water, SKU: SCT123). Because I need it to stain the complex of DNA and proteins, if there is SDS in the dye, it won't work. So, I ask you a question. Does this dye contain any SDS?
1 answer-
This product does not contain SDS.
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