SCT124

GelGreen^® Nucleic Acid Stain 10000X DMSO

GelGreen is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.

• Synonym(s): Fluorescent DNA gel stain
• UNSPSC Code: 12352106
• NACRES: NA.52

Properties

• form: liquid
• technique(s): PCR: suitable
• solubility: water: miscible
• suitability: suitable for nucleic acid staining
• detection method: fluorometric

Description

General description

GelGreen is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GelGreen has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm (Figure 1). Thus, GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader). GelGreen is far more sensitive than SYBR Safe (Figure 2). Unlike SYBR dyes, which are known to be unstable, GelGreen is very stable, both hydrolytically and thermally.

The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelGreen successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelGreen is not classified as hazardous waste.

Spectral Properties
Absorbance: Standard Transilluminator (254 nm) such as a Dark Reader
Emission: Long path green filter such as a SYBR filter or GelStar filter.

Application

GelGreen is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.

Nucleic acid detection

Research Category
Cell Culture

Research Sub Category
Live Cell Dye

Physical form

Liquid

Preparation Note

GelGreen is a very stable dye. Store GelGreen at room temperature, protected from light. Dye precipitation may occur at lower temperatures, resulting in lower signal or the appearance of precipitate on the surface of the gel. If this occurs, heat the solution to 45-50°C for two minutes and vortex. Protect From Light.

Analysis Note

Spectral Properties
Absorbance: Standard Transilluminator (254 nm) such as a Dark Reader
Emission: Long path green filter such as a SYBR filter or GelStar filter.

Legal Information

GelGreen is a registered trademark of Biotium Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 188.6 °F - (refers to pure substance)
• Flash Point(C): 87 °C - (refers to pure substance)