The solubility of this product has been tested in ethanol at 1 mg/mL, and heating to 37°C may be required for complete solubilization. The solubility in DMSO has not been tested, but various sources indicate that this product is soluble in DMSO at a minimum of 20 mg/mL.
H0135
Hydrocortisone
γ-irradiated, powder, BioXtra, suitable for cell culture
Synonym(s):
11β,17α,21-Trihydroxypregn-4-ene-3,20-dione, 17-Hydroxycorticosterone, 4-Pregnene-11β,17α,21-triol-3,20-dione, Cortisol, Kendall’s compound F, Reichstein’s substance M
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1 MG
CZK 1,410.00
About This Item
Empirical Formula (Hill Notation):
C21H30O5
CAS Number:
Molecular Weight:
362.46
UNSPSC Code:
12352209
NACRES:
NA.77
PubChem Substance ID:
EC Number:
200-020-1
Beilstein/REAXYS Number:
1354819
MDL number:
Biological source:
synthetic (organic)
Form:
powder
Assay:
≥80% (HPLC)
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Product Name
Hydrocortisone, γ-irradiated, powder, BioXtra, suitable for cell culture
InChI key
JYGXADMDTFJGBT-VWUMJDOOSA-N
InChI
1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
SMILES string
[H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO
biological source
synthetic (organic)
sterility
γ-irradiated
product line
BioXtra
assay
≥80% (HPLC)
form
powder
technique(s)
cell culture | mammalian: suitable
mp
211-214 °C (lit.)
solubility
ethanol: 1 mg/mL, clear
shipped in
ambient
storage temp.
room temp
Quality Level
Gene Information
human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)
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1 of 4
This Item | H0888 | H3160 | H6909 |
|---|---|---|---|
| biological source synthetic (organic) | biological source soybean | biological source plant (wild yam) | biological source non-animal source |
| assay ≥80% (HPLC) | assay ≥98% | assay 97.0-102.0% dry basis | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 500 |
| product line BioXtra | product line BioReagent | product line - | product line BioXtra |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| form powder | form powder | form powder | form liquid |
Application
Hydrocortisone is used for the following applications:
- Used in myogenic induction and identification[1]
- Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)[2]
- Used in adipogenic differentiation (one of the components in adipogenic medium)[3]
- Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components[4]
- The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone[5]
- Used in differentiation assays of melanoma spheroid cells[6]
Biochem/physiol Actions
Primary glucocorticoid secreted by the adrenal cortex.
Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.
General description
Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.
Physical form
powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
Preparation Note
To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.
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signalword
Danger
hcodes
Hazard Classifications
Repr. 1A - STOT RE 2
target_organs
Adrenal gland,Bone marrow
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Clinical cancer research : an official journal of the American Association for Cancer Research, 5(9), 2511-2519 (1999-09-28)
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In spite of the global consumption of mushrooms, only two epidemiological studies demonstrated an inverse correlation between mushroom intake and the risk of cancer. Therefore, in the present study we evaluated whether extracts from edible mushrooms Agaricus bisporus (portabella), Flammulina
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Could I use DMSO as the solvent for hydrocortisone ?
1 answer-
Helpful?
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How long is the 50 μg/mL stock solution (1 mL absolute ethanol + 19 mL sterile medium) stable when stored in frozen aliquots at -20°C?
1 answer-
Unfortunately, the solution stability of this product at -20°C is not determined internally. However, various sources suggest frozen aliquots stored at -20°C are stable for at least 3-4 months. It is strongly recommend to protect the solution from light.
Helpful?
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What are the recommended storage conditions for stock solutions of item H0135, Hydrocortisone?
1 answer-
The stock solutions can be stored at 4°C and are stable for up to 1 month.
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