The product is supplied as a powder.
Rhodamine B Isothiocyanate can be used for various applications. The only suitability test performed during quality control testing is for labelling efficiency with Bovine Serum Albumin. There is no suitability testing or protocol available for conjugating chitosan polymers. A search of the literature will be required to locate a protocol.
R1755
Rhodamine B isothiocyanate
BioReagent, mixed isomers, suitable for protein labeling
Synonym(s):
RBITC
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100 MG
CZK 4,540.00
500 MG
CZK 13,900.00
1 G
CZK 26,000.00
About This Item
Empirical Formula (Hill Notation):
C29H30ClN3O3S
CAS Number:
Molecular Weight:
536.08
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352116
EC Number:
253-248-9
MDL number:
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Product Name
Rhodamine B isothiocyanate, BioReagent, mixed isomers, suitable for protein labeling
InChI key
YVSWPCCVTYEEHG-UHFFFAOYSA-N
InChI
1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H
SMILES string
[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1
description
labeling efficiency with bovine albumin > 70%
product line
BioReagent
Quality Level
form
powder
mol wt
536.08 g/mol
composition
9-[2-carboxy-5(or 6)-isothiocyanatophenyl]-3,6-bis(diethylamino)xanthylium chloride, ≤100%
color
purple to red-brown
solubility
methanol: 10 mg/mL (dark red to very dark red and dark purple to very dark purple)
fluorescence
λex 543 nm; λem 580 nm in methanol
suitability
suitable for protein labeling
storage temp.
−20°C
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Related Categories
1 of 4
This Item | 283924 | 83689 | R9379 |
|---|---|---|---|
| suitability suitable for protein labeling | suitability - | suitability - | suitability - |
| fluorescence λex 543 nm; λem 580 nm in methanol | fluorescence - | fluorescence λex 553 nm; λem 627 nm in methanol | fluorescence - |
| storage temp. −20°C | storage temp. room temp | storage temp. - | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line - | product line - | product line - |
| description labeling efficiency with bovine albumin > 70% | description - | description - | description - |
Application
General description
Rhodamine B isothiocyanate (RhBITC) is an organic fluorescence dye and is widely used as a fluorescent tag in a variety of biological applications.[5] RhBITC is adsorbed onto Ag on silica or polystyrene beads. It exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol. They are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules.[6]
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Kwan Kim et al.
Biosensors & bioelectronics, 24(7), 1864-1869 (2008-11-05)
Rhodamine B isothiocyanate (RhBITC) is a prototype dye molecule that is widely used as a fluorescent tag in a variety of biological applications. We report in this work that once RhBITC is adsorbed onto Ag on silica or polystyrene beads
Control Over Aragonite Crystal Nucleation and Growth: An In Vitro Study of Biomineralization.
Yael Levi et al.
Chemistry (Weinheim An Der Bergstrasse, Germany), 4 (1998)
Jun-Sung Kim et al.
Journal of veterinary science, 7(4), 321-326 (2006-11-16)
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery
S K Williams et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(4), 2393-2397 (1981-04-01)
Microvessels isolated from rat epididymal fat exhibit differential vesicular ingestion rates for unmodified and non-enzymatically glycosylated rat albumin. While unmodified rat albumin is excluded from ingestion by endothelial micropinocytic vesicles, glycosylated albumin is avidly taken up by endocytosis. Interaction of
Mengying Zhang et al.
Lab on a chip, 10(9), 1199-1203 (2010-04-15)
We report a simple methodology to fabricate PDMS multi-layer microfluidic chips. A PDMS slab was surface-treated by trichloro (1H,1H,2H,2H-perfluorooctyl) silane, and acts as a reusable transferring layer. Uniformity of the thickness of the patterned PDMS layer and the well-alignment could
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Hello, which form does this product come in? powder or liquid please. I would also like to know if it can be conjugated with chitosan polymers (nanomicelles)
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