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Merck

53992-U

Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 5 cm × 300 μm HPLC Capillary Column

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1 EA

CZK 23,700.00

CZK 23,700.00


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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

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Product Name

Ascentis® Express C18, 2.7 Micron Capillary HPLC Column, 2.7 μm particle size, L × I.D. 5 cm × 300 μm

material

stainless steel column

agency

suitable for USP L1

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

400 bar pressure (5801 psi)
60 °C temp. range

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

5 cm × 300 μm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

Quality Level

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1 of 4

This Item
53998-U54271-U54273-U
matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

L × I.D.

5 cm × 300 μm

L × I.D.

5 cm × 500 μm

L × I.D.

15 cm × 300 μm

L × I.D.

15 cm × 500 μm

particle size

2.7 μm

particle size

2.7 μm

particle size

2.7 μm

particle size

2.7 μm

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

technique(s)

HPLC: suitable, UHPLC-MS: suitable, LC/MS: suitable, UHPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis
The capillary housing for Ascentis® Express capillary columns is PEEK with stainless steel end fittings.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within
Francesco Pio Prencipe et al.
Journal of chromatography. A, 1349, 50-59 (2014-05-27)
The study was aimed at developing a new analytical method for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L. (hop), together with a simple extraction procedure. Different extraction techniques, including maceration, heat reflux extraction (HRE), ultrasound-assisted extraction (UAE)
Ahmed Abrahim et al.
Journal of pharmaceutical and biomedical analysis, 51(1), 131-137 (2009-09-18)
Fused-core silica stationary phases represent a key technological advancement in the arena of fast HPLC separations. These phases are made by fusing a 0.5 microm porous silica layer onto 1.7 microm nonporous silica cores. The reduced intra-particle flow path of
Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
Heena et al.
Journal of chromatographic science, 53(5), 800-806 (2014-10-18)
This study represents a new analytical high-performance liquid chromatography-fluorescence detector method for the determination of Al(III) as Al(III) complex with 8-hydroxyquinoline-5-sulfonic acid in a tap water sample and a coke sample. A micellar liquid chromatographic method is proposed for the

Related Content

Ascentis® Express Nano/Capillary Column Care & Use Sheet

Questions

1–5 of 5 Questions  
  1. What is the column housing material for the Ascentis Express, 300 um x 5 cm (53992-U) capillary LC column? Is it a stainless steel column housing, or is it made of another material such as PEEK or fused silica? Additionally, if it is steel, are there other capillary columns available with metal-free housings?

    1 answer
    1. The column housing for the Ascentis Express, 300 um x 5 cm (53992-U) is made of PEEK with stainless steel end fittings.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. What is the pressure rating for 300 μm I.D. Ascentis® Express Capillary HPLC columns?

    1 answer
    1. The 300 μm I.D. Ascentis® Express Capillary HPLC columns are stable to operating pressures up to 6500 psi.

      Helpful?

  4. Can I use Ascentis Express on a UHPLC system?

    1 answer
    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      Helpful?

  5. What is the carbon load for Ascentis® Express C18 HPLC Columns?

    1 answer
    1. Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared

      Helpful?

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