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54273-U
Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns
L × I.D. 15 cm × 500 μm HPLC Capillary Column
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CZK 30,700.00
CZK 30,700.00
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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Product Name
Ascentis® Express C18, 2.7 Micron Capillary HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 500 μm
material
stainless steel column
agency
suitable for USP L1
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
400 bar pressure (5801 psi)
60 °C temp. range
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
15 cm × 500 μm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform
superficially porous particle
matrix active group
C18 (octadecyl) phase
particle size
2.7 μm
pore size
90 Å pore size
operating pH
2-9
application(s)
food and beverages
separation technique
reversed phase
Quality Level
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Related Categories
1 of 4
This Item | 53992-U | 53998-U | 54271-U |
|---|---|---|---|
| matrix active group C18 (octadecyl) phase | matrix active group C18 (octadecyl) phase | matrix active group C18 (octadecyl) phase | matrix active group C18 (octadecyl) phase |
| particle size 2.7 μm | particle size 2.7 μm | particle size 2.7 μm | particle size 2.7 μm |
| L × I.D. 15 cm × 500 μm | L × I.D. 5 cm × 300 μm | L × I.D. 5 cm × 500 μm | L × I.D. 15 cm × 300 μm |
| separation technique reversed phase | separation technique reversed phase | separation technique reversed phase | separation technique reversed phase |
| matrix Fused-Core particle platform, superficially porous particle | matrix Fused-Core particle platform, superficially porous particle | matrix Fused-Core particle platform, superficially porous particle | matrix Fused-Core particle platform, superficially porous particle |
| product line Ascentis® | product line Ascentis® | product line Ascentis® | product line Ascentis® |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within
Francesco Pio Prencipe et al.
Journal of chromatography. A, 1349, 50-59 (2014-05-27)
The study was aimed at developing a new analytical method for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L. (hop), together with a simple extraction procedure. Different extraction techniques, including maceration, heat reflux extraction (HRE), ultrasound-assisted extraction (UAE)
Ahmed Abrahim et al.
Journal of pharmaceutical and biomedical analysis, 51(1), 131-137 (2009-09-18)
Fused-core silica stationary phases represent a key technological advancement in the arena of fast HPLC separations. These phases are made by fusing a 0.5 microm porous silica layer onto 1.7 microm nonporous silica cores. The reduced intra-particle flow path of
Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
Heena et al.
Journal of chromatographic science, 53(5), 800-806 (2014-10-18)
This study represents a new analytical high-performance liquid chromatography-fluorescence detector method for the determination of Al(III) as Al(III) complex with 8-hydroxyquinoline-5-sulfonic acid in a tap water sample and a coke sample. A micellar liquid chromatographic method is proposed for the
Related Content
Ascentis® Express Nano/Capillary Column Care & Use Sheet
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What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
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What is the carbon load for Ascentis® Express C18 HPLC Columns?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
Helpful?
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Can I use Ascentis Express on a UHPLC system?
1 answer-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
Helpful?
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What is the pressure rating for 500 μm I.D. Ascentis® Express Capillary HPLC columns?
1 answer-
The 500 μm Ascentis® Express Capillary HPLC columns are stable to operating pressures up to 5000 psi.
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