While cell culture use is not part of the validation process for the AGE-BSA, independent laboratories have used the AGE-BSA to induce hypoxic stress. The following references may be useful:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C22&q=%22121800%22++%22oxidative+stress%22&btnG=
121800-M
Advanced Glycation Endproduct-BSA
AGE-BSA has been reported to induce apoptosis in cultured human umbilical vein endothelial cells and inhibit nitric oxide synthase activity in proximal tubular epithelial cells.
Sinónimos:
AGE-BSA
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Código UNSPSC:
12352202
NACRES:
NA.25
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Servicio técnico
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Permítanos ayudarleNivel de calidad
Ensayo
≥95% (SDS-PAGE)
Formulario
liquid
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
avoid repeated freeze/thaw cycles
temp. de almacenamiento
−70°C
Descripción general
Prepared by reacting BSA with glycoaldehyde under sterile conditions. Glycated-BSA shows a 5,000 - 10,000% increase in fluorescence as compared to normal BSA (confirmed by fluorescence spectrophotometry, excitation/emission 370/440 nm). AGE-BSA has been reported to induce apoptosis in cultured human umbilical vein endothelial cells and inhibit nitric oxide synthase activity in proximal tubular epithelial cells. Advanced glycation end products and their receptors have been implicated in the pathogenesis of diabetes, induction of proinflammatory cytokines, and stimulation of smooth muscle proliferation, and fibronectin production.
Prepared by reacting bovine serum albumin (BSA) with glycoaldehyde under sterile conditions. Fluorescence of AGE-BSA is confirmed by fluorescence spectrophotometry, excitation/emission = 370/440 nm. Glycated BSA shows a 5000-10,000% increase in fluorescence compared to control BSA. AGE and their receptors have been implicated in the pathogenesis of diabetes, induction of pro-inflammatory cytokines, and stimulation of smooth muscle proliferation and fibronectin production. AGE-BSA has also been shown to induce apoptosis in cultured human umbilical vein endothelial cells (HUVEC) and inhibit nitric oxide synthase activity in proximal tubular epithelial cells of the kidney.
Forma física
In sterile-filtered PBS.
Otras notas
Okamoto, T., et al. 2002. Microvasc. Res.63, 186.
Ohgami, N., et al. 2001. J. Biol. Chem.276, 3195.
Wang, R., et al. 2001. J. Nippon Med. Sch.68, 472.
Sakata, N., et al. 2000. J. Atheroscler. Thromb.7, 169.
Verbeke, P., et al. 2000. Biochim. Biophys. Acta1502, 481.
Farboud, B., et al. 1999. Mol. Vis.5, 11.
Huang, J.-S., et al. 1999. Biochem. J.342, 231.
Min, C., et al. 1999. Diabetes Res. Clin. Pract.46, 197.
Neumann, A., et al. 1999. FEBS Lett.453, 283.
Stitt, A.W., et al. 1999. Biochem. Biophys. Res. Commun.256, 549.
Nazaimoon, W. and Bak, K. 1998. Malays. J. Pathol.20, 83.
Ohgami, N., et al. 2001. J. Biol. Chem.276, 3195.
Wang, R., et al. 2001. J. Nippon Med. Sch.68, 472.
Sakata, N., et al. 2000. J. Atheroscler. Thromb.7, 169.
Verbeke, P., et al. 2000. Biochim. Biophys. Acta1502, 481.
Farboud, B., et al. 1999. Mol. Vis.5, 11.
Huang, J.-S., et al. 1999. Biochem. J.342, 231.
Min, C., et al. 1999. Diabetes Res. Clin. Pract.46, 197.
Neumann, A., et al. 1999. FEBS Lett.453, 283.
Stitt, A.W., et al. 1999. Biochem. Biophys. Res. Commun.256, 549.
Nazaimoon, W. and Bak, K. 1998. Malays. J. Pathol.20, 83.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Toxicity: Standard Handling (A)
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Hello, I am working on detecting and eventually reducing Oxidative stress in diabetes in vitro. I am using SHSY5Y and RT4 D6P2T cells. Can I use this product to immitate the diabetic condition (presence of AGE) ideally leading to oxidative stress?
1 respuesta-
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What compounds are present in this product 121800-M (AGE-BSA)? It is a mixture of different AGEs? In paper, DOI: 10.1038/srep27848 it is provided that it is a `mix of Nε -(carboxymethyl)-lysine,pentosidine, and others`. Could you please clarify this?
1 respuesta-
This product is prepared by reacting bovine serum albumin with glycoaldehyde, resulting in a glycated BSA with a 5,000–10,000% increase in fluorescence (excitation/emission = 370/440 nm) compared to control BSA, as confirmed by fluorescence spectrophotometry. The range of modifications is not determined using mass spectrometry. End products or impurities have not been further characterized.
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What is the molecular weight of AGE-BSA, 121800-M?
1 respuesta-
The predicted molecular weight of AGE-BSA is approximately 66.5 kDa.
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What is the concentration of the product supplied? 121800-10MG
1 respuesta-
The concentration of the item is 10 mg/mL. This value is reported in the lot-specific Certificate of Analysis.
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If the diluted AGE-RAGE is prepared a year ago and was stored in -60 degree, can it still be used?
1 respuesta-
Unfortunately, the solution stability has not been determined. However, other sources state that following reconstitution, this chemical is stable at -20 to -70 °C for only 3 months.
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What is the molecular weight of the product 121800-M (AGE-BSA)? and what is the degree of glycation?
1 respuesta-
AGE-BSA has a predicted MW of ~66.5 kDa. The degree of glycation is not determined.
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