OP145

Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11)

liquid, clone 4B2C1.11, Calbiochem^®

• Sinónimos: Anti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 4B2C1.11, monoclonal
• Formulario: liquid
• no contiene: preservative
• reactividad de especies: human
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (2 µg/mL, chemiluminescence); Immunofluorescence (2.5 µg/mL); Immunoprecipitation (1 µg/reaction, ; Paraffin Sections (2.5 µg/mL, heat pre-treatment required))
• isotipo: IgG1
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MDM2(4193)

Descripción

Descripción general

Purified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein.

Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 and MCF-7 cells and breast carcinoma tissue.

This Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-5).

Inmunógeno

Human

recombinant, human MDM2

Aplicación

Immunoblotting (2 g/ml, chemiluminescence)
Immunofluorescence (2.5 g/ml)
Immunoprecipitation (1 g/reaction, see application references)
Paraffin Sections (2.5 g/ml, heat pre-treatment required)

Envase

Please refer to vial label for lot-specific concentration.

Forma física

In 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5.

Nota de preparación

Following initial thaw, aliquot and freeze (-20°C).

Nota de análisis

Positive Control
A549 or MCF7 cells or breast carcinoma tissue

Otras notas

Antibody should be titrated for optimal results in individual systems.

Marchetti, A., et al. 1995. J. Pathol.175, 31.
Barak, Y., et al. 1993. EMBO. J.12, 461.
Ladanyi, M., et al. 1993. Cancer Res.53, 16.
Leach, F.S., et al. 1993. Cancer Res.53, 2231.
Oliner, J.D., et al. 1993. Nature362, 857.
Momand, J., et al. 1992. Cell69, 1237.
Oliner, J.D., et al. 1992. Nature358, 80.
Fakharzadeh, S.S., et al. 1991. EMBO J.10, 1565.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Toxicity: Standard Handling (A)

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3