Merck
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B3773

Sigma-Aldrich

Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal

clone HP-6017, purified from hybridoma cell culture

Sinónimos:
Monoclonal Anti-Human IgG (Fc specific)
NACRES:
NA.46

Nivel de calidad

200

origen biológico

mouse

conjugado

biotin conjugate

forma del anticuerpo

purified immunoglobulin

antibody product type

secondary antibodies

clon

HP-6017, monoclonal

formulario

buffered aqueous solution

species reactivity

sheep, horse (IgG), rabbit, goat, human

technique(s)

direct ELISA: 1:80,000

isotipo

IgG2a

enviado en

dry ice

temp. de almacenamiento

−20°C

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Descripción general

Immunoglobulin IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Immunoglobulin super-family have a common structure, comprising of two heavy (H) chains and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.

Especificidad

The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study.

Aplicación

Anti-Human IgG (Fc specific) Biotin antibody, Mouse monoclonal has been used in fluoroimmunoassay of plasma samples.
Monoclonal Anti-Human IgG (Fc specific)-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:80,000. It was used for labeling mouse splenocytes and analysis by flow cytometry.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Acciones bioquímicas o fisiológicas

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG (Fc specific) antibody allows for the binding of various labels such as avidin or streptavidin.
IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions, and IgG4 is associated with asymptomatic infection. Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG generates fragment crystallisable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Introduzca el número de lote para buscar un certificado de análisis (COA).

Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

Quotes and Ordering

Intracellular cytokines may model immunoregulation of abacavir hypersensitivity in HIV-infected subjects.
King D, et al.
The Journal of Allergy and Clinical Immunology, 115(5), 1081-1087 (2005)
C Whitney et al.
Infection and immunity, 60(6), 2194-2200 (1992-06-01)
Porphyromonas gingivalis is a suspected pathogen in rapidly progressive periodontitis (RPP). We have determined the anti-P. gingivalis serum immunoglobulin G (IgG) isotype response and avidity and the subclass titer distributions for 30 RPP patients and 30 age-, sex-, and race-matched
Deborah King et al.
The Journal of allergy and clinical immunology, 115(5), 1081-1087 (2005-05-04)
The clinical treatment of patients with HIV and adverse drug events may be enhanced by an understanding of the underlying mechanisms. About 4% of patients with HIV receiving the potent antiretroviral drug abacavir develop a hypersensitivity reaction. This idiosyncratic reaction
Ioana Carcea et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(45), 15317-15329 (2010-11-12)
Cortical efferents growing in the same environment diverge early in development. The expression of particular transcription factors dictates the trajectories taken, presumably by regulating responsiveness to guidance cues via cellular mechanisms that are not yet known. Here, we show that
Gunnveig Grødeland et al.
PloS one, 8(11), e80008-e80008 (2013-11-19)
Different diseases require different immune responses for efficient protection. Thus, prophylactic vaccines should prime the immune system for the particular type of response needed for protection against a given infectious agent. We have here tested fusion DNA vaccines which encode

Artículos

Anti-Human Antibodies for COVID-19 (SARS-CoV-2) Immunoglobulin Detection Research

Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.

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