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B3773

Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal

clone HP-6017, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human IgG (Fc specific)

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0.2 mL
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$222.00
0.5 mL
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$552.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
HP-6017, monoclonal
Application:
ELISA (d)
Citations:
16

$222.00

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biological source

mouse

Quality Level

200

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

HP-6017, monoclonal

form

buffered aqueous solution

species reactivity

rabbit, sheep, horse (IgG), goat, human

technique(s)

direct ELISA: 1:80,000

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Immunoglobulin IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Immunoglobulin super-family have a common structure, comprising of two heavy (H) chains and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.

Application

Anti-Human IgG (Fc specific) Biotin antibody, Mouse monoclonal has been used in fluoroimmunoassay of plasma samples.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Monoclonal Anti-Human IgG (Fc specific)-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:80,000. It was used for labeling mouse splenocytes and analysis by flow cytometry.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG (Fc specific) antibody allows for the binding of various labels such as avidin or streptavidin.
IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions, and IgG4 is associated with asymptomatic infection. Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG generates fragment crystallisable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.
The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal clone HP-6017, purified from hybridoma cell culture

Sigma-Aldrich

B3773

Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal

Anti-Human IgG2−Biotin antibody, Mouse monoclonal clone HP-6014, purified from hybridoma cell culture

Sigma-Aldrich

B3398

Anti-Human IgG2−Biotin antibody, Mouse monoclonal

Anti-Human IgG1−Biotin antibody, Mouse monoclonal clone 8c/6-39, purified from hybridoma cell culture

Sigma-Aldrich

B6775

Anti-Human IgG1−Biotin antibody, Mouse monoclonal

Anti-Human IgG3−Biotin antibody, Mouse monoclonal clone HP-6050, purified from hybridoma cell culture

Sigma-Aldrich

B3523

Anti-Human IgG3−Biotin antibody, Mouse monoclonal

technique(s)

direct ELISA: 1:80,000

technique(s)

direct ELISA: 1:30,000

technique(s)

direct ELISA: 1:1,000

technique(s)

direct ELISA: 1:40,000

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

species reactivity

rabbit, sheep, horse (IgG), goat, human

species reactivity

human

species reactivity

-

species reactivity

human

isotype

IgG2a

isotype

IgG1

isotype

IgG2a

isotype

IgG1

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000545313
0000531106
0000545313
0000531106
0000519082

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Related Content

Marta Baranowska et al.
Vaccine, 33(49), 6988-6996 (2015-09-22)
Vaccination is at present the most efficient way of preventing influenza infections. Currently used inactivated influenza vaccines can induce virus-neutralizing antibodies that are protective against a particular influenza strain, but hamper the induction of cross-protective T-cell responses to later infections.
Divya Thiagarajan et al.
Journal of immunology (Baltimore, Md. : 1950), 205(8), 2109-2116 (2020-09-06)
Abs against phosphorylcholine (anti-PC) and Abs against malondialdehyde (anti-MDA) may be protective in chronic inflammation, like atherosclerosis and cardiovascular disease. It is not known how they develop early in life. Ab titers were measured using ELISA in healthy women (n
Intracellular cytokines may model immunoregulation of abacavir hypersensitivity in HIV-infected subjects.
King D, et al.
The Journal of Allergy and Clinical Immunology, 115(5), 1081-1087 (2005)
View All Related Papers

Global Trade Item Number

SKUGTIN
B3773-.2ML04061833428399
B3773-.5ML04061837514852

Questions

  1. At what concent the 2 ML B3773 Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal is provided

    1 answer

    1. The IgG concentration of this product is lot-specific and may range from 2.0 - 4.0 mg/mL. This information is reported in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/b3773#product-documentation

      Please see the following link to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/237/578/b3773dat.pdf

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