The endotoxin is not determined for this product. Please see the links below to review similar products that are endotoxin tested:
https://www.sigmaaldrich.com/product/sigma/g1393
https://www.sigmaaldrich.com/product/aldrich/918644
ES-006
EmbryoMax® Gelatin Solution
0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.
Synonym(s):
Porcine Gelatin Coating Solution, porcine gelatin
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75
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Product Name
EmbryoMax® 0.1% Gelatin Solution, The EmbryoMax 0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.
form
liquid
manufacturer/tradename
Specialty Media
EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
1 of 4
This Item | SF008 | 918636 | G6144 |
|---|---|---|---|
| technique(s) cell culture | stem cell: suitable | technique(s) cell culture | stem cell: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form viscous liquid | form - |
| Quality Level 100, 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| manufacturer/tradename Specialty Media | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
| input sample type induced pluripotent stem cell(s) | input sample type induced pluripotent stem cell(s) | input - | input - |
Application
Procedure for Coating Multiwell Dishes
1. Add the appropriate amounts of 0.1% Gelatin in Sterile Water to each well of the plate as indicated in the table below:
2. Leave the gelatin solution on the wells for at least 30 minutes at room temperature, with dish lids on.
3. Remove the gelatin solution and discard.
4. Immediately add cells to the dish.
Note: Do not allow the dishes to dry before adding cells.
1. Add the appropriate amounts of 0.1% Gelatin in Sterile Water to each well of the plate as indicated in the table below:
| Wells | _ | Amount/Well |
|---|---|---|
| 96 | 100 μL | |
| 48 | 300 μL | |
| 24 | 0.5 mL | |
| 12 | 1.0 mL | |
| 6 | 1.5-2.0 mL |
2. Leave the gelatin solution on the wells for at least 30 minutes at room temperature, with dish lids on.
3. Remove the gelatin solution and discard.
4. Immediately add cells to the dish.
Note: Do not allow the dishes to dry before adding cells.
Physical form
EmbryoMax 0.1% Gelatin Solution. Gelatin is sourced from porcine. Product is ready to use for plating.
Preparation Note
Store at ambient temperature for upto 2 years
Legal Information
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
Protocols
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.
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Is this product endotoxin-free?
1 answer-
Helpful?
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Is this product composed of Type A or Type B gelatin?
1 answer-
The gelatin type used for product ES-006-B is type A.
Helpful?
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if we are using treated flasks (like crystal-clear polystyrene flasks or Poly-D- lysine coated flasks) then still we have to use gelatin or not?
1 answer-
Whether the need to use gelatin in cell culture will depend on the specific requirements of the cells and the experimental goals. Gelatin is often used as a coating material to enhance cell adhesion in some cell culture applications even with cell culture treated flasks.
To further discuss, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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