MABS2044

Anti-HPd2 Antibody, clone DHIC3-5H10

clone DHIC3-5H10, from mouse

• Synonym(s): Human pancreatics duct cells
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: DHIC3-5H10, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μg
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable (paraffin)
• isotype: IgMκ
• target post-translational modification: unmodified

Description

General description

Pancreas performs both the endocrine and the exocrine function. The bulk of pancreas is composed of exocrine cells that produce digestion enzymes which are released into ducts to be emptied into the duodenum. The endocrine pancreas is composed of islets of Langerhans that produce hormones. At least five distinct endocrine cell types have been identified in the endocrine pancreas, each producing a different hormone. Beta cells produce insulin, which is considered as an anabolic hormone and regulates blood glucose levels and is also involved in fat and protein synthesis. Alpha cells produce glucagon that acts when blood glucose levels are low. Ductal cells of pancreas form the epithelial lining of the branched tubes that deliver digestive enzymes into the duodenum. They also secrete bicarbonate to neutralize acidic contents coming in from stomach. Duct cells may contribute up to 10% of all pancreatic cells. Acinar cells function as a unit of the exocrine pancreas and they synthesize, store, and secrete digestive enzymes (lipase, alpha amylase, trypsin, and chymotrypsin), which under normal physiological conditions are activated only after reaching the duodenum. Premature activation of these enzymes within pancreatic acinar cells can lead to acute pancreatitis. Clone DCHI3-5H10 can be used to detect duct cells in human pancreatic tissue. (Ref.: Dorrell, C., et al. (2008). Stem Cell Research (2008). 1(3); 183-194; Grapin-Botton, A (2005). Int. J. Biochem. Cell Biol. 37(3); 504-510; Leung, PS (2006). Int. J. Biochem. Cell Biol. 38(7); 1024-1030).

Immunogen

Trypsin-dissociated human pancreatic duct cells.

Application

Anti-HPd2, clone DHIC3-5H10, Cat. No. MABS2044, is a mouse monoclonal antibody that detects Duct2 cells of pancreas and has been tested for use in Immunofluorescence and Immunohistochemistry (Paraffin).

Immunofluorescence Analysis: A representative lot detected HPd2 in Immunofluorescence applications (Dorrell, C., et. al. (2008). Stem Cell Res. 1(3):183-94).

Research Category
Signaling

Biochem/physiol Actions

Clone DCHI3-5H10 specifically detects human pancreatic duct cells.

Physical form

Format: Purified

Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunohistochemistry (Paraffin) in human pancreatic tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HPd2 in human pancreatic tissue sections.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable