62970

Lysozyme from chicken egg white

dialyzed, lyophilized, powder, ~100000 U/mg

• Synonym(s): Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS Number: 12650-88-3
• EC Number: 3.2.1.17 (BRENDA, IUBMB)
• EC Number: 235-747-3
• MDL number: MFCD00131557
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: chicken egg white
• Quality Level: 100
• form: powder
• quality: dialyzed; lyophilized
• specific activity: ~100000 U/mg
• mol wt: single-chain 14.3 kDa; M_r ~14600
• technique(s): cell based assay: suitable
• suitability: suitable for cell lysis
• UniProt accession no.: P00698
• application(s): cell analysis
• storage temp.: 2-8°C
• Gene Information: chicken ... LYZ(396218)

Description

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Lysozyme from chicken egg white has been used:

• as a standard in small-angle neutron scattering experiments [1] and infrared absorbance spectroscopy[2]
• as a constituent of cell lysis buffer[3]
• in protein crystallization experiments[4]

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.

Other Notes

1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)

Suitable for the hydrolysis of bacterial cell walls, mucopolysaccharides, mucopolypeptides or chitin; degradability of L. lactis subsp. cremoris H2 cell walls.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves