Yes, this product is soluble in water at 50 mg/mL at 20 °C.
C6158
Crystal Violet
≥90% purity anhydrous basis, ACS reagent, powder
Synonym(s):
Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
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About This Item
Linear Formula:
C25H30N3Cl
CAS Number:
Molecular Weight:
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
208-953-6
MDL number:
Colour Index Number:
42555
Beilstein/REAXYS Number:
3580948
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Product Name
Crystal Violet, ACS reagent, ≥90.0% anhydrous basis
InChI key
ZXJXZNDDNMQXFV-UHFFFAOYSA-M
InChI
1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
SMILES string
[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
grade
ACS reagent
assay
≥90.0% anhydrous basis
form
powder
loss
≤7.5% loss on drying
color
green to very dark green
pH
2.5-3.5 (20 °C, 10 g/L)
mp
205 °C (dec.) (lit.)
solubility
water: soluble 50 g/L at 27 °C
density
1.19 g/cm3 at 20 °C
absorption
passes test
suitability
passes test for sensitivity as indicator
antibiotic activity spectrum
fungi
application(s)
diagnostic assay manufacturing
hematology
histology
mode of action
cell membrane | interferes
enzyme | inhibits
storage temp.
room temp
Quality Level
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This Item | 32675 | C0775 | 46364 |
|---|---|---|---|
| grade ACS reagent | grade indicator for the determination of the redox potential | grade certified by the Biological Stain Commission | grade analytical standard |
| suitability passes test for sensitivity as indicator | suitability complies for sensitivity | suitability - | suitability - |
| color green to very dark green | color S. No.: 785 | color green to very dark green | color - |
| form powder | form powder | form powder | form - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) cleaning products |
| solubility water: soluble 50 g/L at 27 °C | solubility water: 0.1% | solubility H2O: 1 mg/mL | solubility water: soluble 50 g/L at 27 °C |
Application
Biochem/physiol Actions
Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA.[3] Crystal violet is also used for the staining of bacteria in gram staining technique.[4] It is also used for the staining of plant chromosomes.[5] Crystal violet helps in colorimetric measurement of cell viability.[6]
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Plant Chromosomes (1999)
The activation of c-Jun NH2-terminal kinase is required for dihydroartemisinin-induced autophagy in pancreatic cancer cells.
Jia G, et al.
Journal of Experimental & Clinical Cancer Research, 33, 8-8 (2014)
Downregulation of survivin expression exerts antitumoral effects on mouse breast cancer cells in vitro and in vivo.
Ma WH, et al.
Oncology Letters, 11, 159-159 (2016)
A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet.
Ishiyama M, et al.
Biological & Pharmaceutical Bulletin, 19, 1518-1518 (1996)
Eric H Clifton et al.
PloS one, 13(3), e0194815-e0194815 (2018-03-23)
Terrestrial plants can harbor endophytic fungi that may induce changes in plant physiology that in turn affect interactions with herbivorous insects. We evaluated whether the application of entomopathogenic fungi Beauveria bassiana and Metarhizium brunneum to soybean seeds could become endophytic
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