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Merck

E6779

EZview™ Red Anti-HA Affinity Gel

Synonym(s):

Anti-HA

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UNSPSC Code:
12352203
NACRES:
NA.56

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shelf life

1 yr at 2‑8 °C

analyte chemical class(es)

proteins (hemagglutinin)

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

matrix

crosslinked agarose

capacity

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

storage temp.

2-8°C

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EZview™ Red Anti-HA Affinity Gel

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technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

-

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

shelf life

1 yr at 2‑8 °C

shelf life

1 yr at 2‑8 °C

shelf life

-

shelf life

-

analyte chemical class(es)

proteins (hemagglutinin)

analyte chemical class(es)

proteins (c-Myc)

analyte chemical class(es)

-

analyte chemical class(es)

proteins

matrix

crosslinked agarose

matrix

-

matrix

-

matrix

(4% agarose bead; 45-165μm bead size)

capacity

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

capacity

~0.05 mg/mL binding capacity

capacity

≥0.6 mg/mL, gel binding capacity

capacity

>0.6 mg/mL, resin binding capacity (FLAG-BAP)

General description

EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition.

Application

EZview™ Red Anti-HA Affinity Gel has been used for immunoblot analysis[1] and immunoprecipitation assay.[2]
Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Biochem/physiol Actions

Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Other Notes

The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000468473
0000468473
0000373135
0000360953
0000360953

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Data Sheet - E6779

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