S5150

Sodium chloride solution

Name: Sodium chloride solution
Brand: SIGMA

5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

Synonym(s):

Halite, NaCl, sodium chloride

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About This Item

Linear Formula:

NaCl

CAS Number:

7647-14-5

Molecular Weight:

58.44

UNSPSC Code:

12352302

NACRES:

NA.25

PubChem Substance ID:

24899641

EC Number:

231-598-3

Beilstein/REAXYS Number:

3534976

MDL number:

MFCD00003477

Grade:

Molecular Biology

Form:

liquid

Key Documents

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Product Name

Sodium chloride solution, 5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

InChI

1S/ClH.Na/h1H;/q;+1/p-1

SMILES string

[Na+].[Cl-]

grade

Molecular Biology

sterility

0.2 μm filtered

product line

BioReagent

form

liquid

concentration

5 M in H₂O

technique(s)

ChIP: suitable
DNA extraction: suitable
cell culture | mammalian: suitable

impurities

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected
<1.0 EU/mL Endotoxin level
<16 cfu/mL Bioburden

pH

6.0-8.0

density

1.190 g/cm³

anion traces

phosphate (PO₄^3-): ≤5 ppm

cation traces

K: ≤50 ppm
heavy metals (as Pb): ≤5 ppm

suitability

suitable for Western blot
suitable for molecular biology

application(s)

agriculture
agriculture

foreign activity

DNAse, Exonuclease; RNAse; NICKase, Endonuclease; Protease, none detected

Quality Level

200

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Show Differences

1 of 4

This Item	59222C	S6546	S0817
Sigma-Aldrich S5150 Sodium chloride solution	SAFC 59222C Sodium chloride solution	Sigma-Aldrich S6546 Sodium chloride solution	Sigma-Aldrich S0817 Sodium chloride solution
form liquid	form liquid	form -	form -
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200
grade for molecular biology	grade -	grade -	grade -
pH 6.0-8.0	pH -	pH -	pH -
anion traces phosphate (PO₄^3-): ≤5 ppm	anion traces -	anion traces -	anion traces -
cation traces K: ≤50 ppm, heavy metals (as Pb): ≤5 ppm	cation traces -	cation traces -	cation traces -

Application

Sodium chloride solution has been used as a component of mycobacterial lysis buffer to extract DNA.[1] It has also been used along with Dulbecco′s phosphate-buffered saline (DPBS) in the preparation of degassed buffer to elute plasma samples.[2]

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Preparation Note

Prepared in 18 megohm water.

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S6150

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71383

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Sodium chloride, Redi-Dri^™, ≥99%, DNase, RNase, and protease (none detected), free-flowing

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000512782

0000511504

0000491114

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Gender-dependent effect of Gpbar1 genetic deletion on the metabolic profiles of diet-induced obese mice

Vassileva G, et al.

The Journal of Endocrinology, 205(3), 225-232 (2010)

Detection and verification of Mycobacterium avium subsp. paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individuals with and without Crohn's disease.

Tim J Bull et al.

Journal of clinical microbiology, 41(7), 2915-2923 (2003-07-05)

Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows'

CarbAcineto NP test for rapid detection of carbapenemase-producing Acinetobacter spp.

Laurent Dortet et al.

Journal of clinical microbiology, 52(7), 2359-2364 (2014-04-25)

Multidrug-resistant Acinetobacter baumannii isolates, particularly those that produce carbapenemases, are increasingly reported worldwide. The biochemically based Carba NP test, extensively validated for the detection of carbapenemase producers among Enterobacteriaceae and Pseudomonas spp., has been modified to detect carbapenemase production in

Effect of prehospital induction of mild hypothermia on survival and neurological status among adults with cardiac arrest: a randomized clinical trial.

Francis Kim et al.

JAMA, 311(1), 45-52 (2013-11-19)

Hospital cooling improves outcome after cardiac arrest, but prehospital cooling immediately after return of spontaneous circulation may result in better outcomes. To determine whether prehospital cooling improves outcomes after resuscitation from cardiac arrest in patients with ventricular fibrillation (VF) and

Heterogeneity in the inter-tumor transcriptome of high risk prostate cancer.

Alexander W Wyatt et al.

Genome biology, 15(8), 426-426 (2014-08-27)

Genomic analyses of hundreds of prostate tumors have defined a diverse landscape of mutations and genome rearrangements, but the transcriptomic effect of this complexity is less well understood, particularly at the individual tumor level. We selected a cohort of 25

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Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

Questions

1–2 of 2 Questions

Anonymous

5 months ago

How can I determine the shelf life / expiration / retest date of this product?

1 answer
1. Technical Support
  
  5 months ago
  
  If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
  For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
  For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
  For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
  
  Helpful?
Anonymous

a year ago

How is shipping temperature determined? And how is it related to the product storage temperature?

1 answer
1. Technical Support
  
  a year ago
  
  Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
  
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