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Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

ReadyBlue Protein Gel Staining Protocols

ReadyBlue™ Protein Gel Stain offers rapid Coomassie® staining for polyacrylamide gels with a simplified protocol.

Introduction to SDS-PAGE - Separation of Proteins Based on Size

Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System

Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Chromatin Isolation by RNA Purification (ChIRP) Protocol

Chromatin Isolation by RNA purification (ChIRP) protocol for isolating RNA-bound genome regions.

Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters

Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.

Chemically Defined Media Supplements Improve Quality and Lower Risk in Upstream Bioprocessing

Chemically defined media supplements offer many benefits including decreased lot-to-lot variation, decreased risks, and improved glycosylation profiles.

Software Simplifies Compliance with 21 CFR Part 11 and EudraLex Good Manufacturing Practice Volume 4 Annex 11

How software can simplify 21 CFR Part 11 and EudraLex Volume 4 Annex 11 compliance and provide additional benefits such as access control, audit trails and easier search and retrieval.

Synthetic Carbon Adsorbents for Host Cell Protein Removal in Monoclonal Antibody Purification

Demonstration of the excellent capability of Carboxen® synthetic carbon adsorbents to reduce host cell proteins (HCPs) and recover monoclonal antibodies (mAbs) using a post protein A pool step.

Value Drivers and Benefits of Closed Processing

Closed processing enables more streamlined, flexible, and cost-effective multi-product and multi-modal facilities, while ensuring the highest level of product quality and safety. Learn more about how contamination risk is mitigated and the additional value and benefits of closed processing.

One-Batch Calibration: A Process-, Instrument-, Scale- & Site-agnostic Method For Streamlining Raman Implementation

Challenges associated with Raman spectroscopy implementation in bioprocesses can be overcome by the innovative one-batch calibration approach. This streamlined process requires less time and resources compared to traditional approaches, unlocking Raman benefits for biopharmaceutical applications.

Cell Harvest, Lysis, Neutralization and Clarification of Plasmid DNA (pDNA) for mRNA, plasmid-based DNA Vaccines, and Viral Vector applications

An overview of the cell harvest, lysis, neutralization, and clarification steps used for pDNA purification along with case study and practical advice.

Chromatographic Purification of Plasmid DNA (pDNA) for mRNA, Plasmid-based DNA Vaccines, and Viral Vector Applications

See case study examples of how to optimize chromatographic purification of plasmid DNA for Biopharmaceutical Applications.

Plasmid DNA Purification Process

Free eBook outlines plasmid DNA manufacturing process and downstream purification challenges for various applications.

Performance Characteristics of the Mobius^® ADC Reactor for Conjugation

ADC production relies on conjugation to link the cytotoxic payload to the mAb. Explore the benefits of a single-use reactor for efficient conjugation.

Improving Efficiency and Control in Biopharma Manufacturing With Process Analytical Technology

Key considerations for process analytical technology (PAT) implementation in biopharma and its role in enabling the facility of the future.

Assay of Betamethasone Sodium Phosphate by HPTLC

Development of an HPTLC based method, using Silica Gel 60 F254 plate, to determine Betamethasone Sodium Phosphate over a concentration range of 1 to 7 µg/spot.

Assay of Doxepin Hydrochloride USP Monograph

The FPP Purospher® STAR RP-8e column offers a reliable and consistent assay with strong peak shapes and linearity, ensuring precise quantification.

Clozapine Assay HPLC Method acc to USP Monograph

An HPLC-UV method for the identification and quantification of clozapine in API samples and tablet formulations, following the USP monograph guidelines.

Determination of Baicalin, Chlorogenic acid and Forsythin

Analyze Shuanghuang Lian oral liquid using Discovery® HS C18 HPLC column per Chinese Pharmacopeia 2020. Quantify baicalin, chlorogenic acid, and forsythin with high accuracy.

API Solubility and Dissolution Enhancement Via Formulation

Our overview of API solubility and dissolution enhancement methods can help you decide between the many options to take during formulation.

Active Pharmaceutical Ingredient (API) Stability in Solid Dose Formulation

This article describes the use of excipients to help address API stability issues.

Improving API Solubility with Mesoporous Silica

Discover how mesoporous silica enhances API solubility, unlocking the full potential of promising drugs with improved bioavailability.

Egg-based and Cell-based Influenza Vaccine Manufacturing Methods

Influenza vaccines are commonly made using egg-based and cell-based manufacturing strategies. Find step-by-step information on the manufacturing process for each method.

Eshmuno^® Fit Custom Affinity Resin Development Services: Frequently Asked Questions

This page answers all the frequently asked questions related to our Eshmuno® Fit custom affinity resin development services.

Antibody Standard Validation

Explore antibody validation, including antibody and target, species validation, lot validation, application validation, storage and handling, citations, and quality control, in detail.

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

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