This DMSO product has not been tested for suitability in HPLC applications. Please see the links below to review DMSO products suitable for HPLC:
https://www.sigmaaldrich.com/product/sigald/34869
https://www.sigmaaldrich.com/product/saj/074871
https://www.sigmaaldrich.com/product/sial/94563
D2650
Dimethyl sulfoxide
Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Dimethyl sulfoxide
Synonym(s):
DMSO
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100 ML
£261.00
£261.00
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About This Item
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.75
Grade:
Hybri-Max™
Assay:
≥99.7%
Technique(s):
cell culture | hybridoma: suitable
cryopreservation: suitable
cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)
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grade
Hybri-Max™
Quality Level
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
sterility
sterile-filtered
product line
BioReagent
Assay
≥99.7%
form
liquid
autoignition temp.
573 °F
expl. lim.
42 %, 63 °F
technique(s)
cell culture | hybridoma: suitable
cryopreservation: suitable
impurities
endotoxin, tested
≤0.1% water
water
refractive index
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: miscible (completely)
density
1.10 g/mL (lit.)
SMILES string
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
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This Item | D2653 | D2438 | D4540 |
|---|---|---|---|
| technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) cell culture | plant: suitable, cryopreservation: suitable |
| Quality Level 400 | Quality Level 300 | Quality Level 400 | Quality Level 200 |
| grade Hybri-Max™ | grade Hybri-Max™ | grade BioPerformance Certified | grade - |
| assay ≥99.7% | assay ≥99.7% | assay - | assay ≥99.5% (GC) |
| solubility H2O: miscible (completely) | solubility H2O: miscible (completely) | solubility H2O: miscible (completely) | solubility H2O: miscible (completely) |
| form liquid | form liquid | form liquid | form liquid |
Application
DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).
Packaging
100 mL in amber bottle
Glass ampoule packaging is now available under a new product number, D2653
Other Notes
This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Disclaimer
Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Overview of good cell banking practices for cell line cryopreservation purposes.
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
Protocols
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.
Human ips cell passaging protocol using EZ-LiFT™ Reagent, an enzyme-free and chemically defined pluripotent stem cell dissociation reagent.
Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
Related Content
Product Information Sheet
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Is this DMSO suitable for HPLC?
1 answer-
Helpful?
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Is this DMSO suitable to be used as a drug solvent in animal studies?
1 answer-
This product has not been tested for use in animal models. However, this is a sterile-filtered DMSO that has been tested for cytotoxicity and is expected to be suitable for in vivo research applications.
Helpful?
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How many days after opening the bottle is DMSO safe to use?
1 answer-
This product is assigned an expiration date of approximately two years from the date of Quality Release. This date is lot specific and available on the product label and Certificate of Analysis. After opening, the material should be stored tightly sealed to prevent moisture uptake. Given proper handling and storage, the product should be stable until the published expiration date.
Helpful?
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I ordered 10 x 10ml vials for fibroblast culture work. My first endeavor in culture domain. What are the storage conditions for DMSO of this grade? This arrived in plain cardboard box at room temperature.
1 answer-
DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots with inert gas and store them in a compatible, tightly sealed container, at a cool storage area, such as 4°C. DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Safety Data Sheet and the Product Information Sheet for compatibility of DMSO with various materials and for further details.
Helpful?
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Is it safe in terms of health and product safety to store a leftover DMSO ampule covered with parafilm in a flammable cabinet?
1 answer-
DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots and store them under inert gas, in a compatible, tightly sealed container, at a cool storage area, such as 4°C.
DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Product Information Sheet and the Safety Data Sheet at the link below for compatibility of DMSO with various materials and for further details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265
Helpful?
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Is this anhydrous?
1 answer-
This product is not considered anhydrous. The maximum allowable water content is 0.1% which is reported on the lot-specific Certificate of Analysis. Please see the link below to review the anhydrous DMSO products available:
https://www.sigmaaldrich.com/search/dmso-anhydrous?focus=products&page=1&perpage=30&sort=relevance&term=DMSO%20anhydrous&type=productHelpful?
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I ordered 10 ml glass vials, used 7 ml, Can I store it in centrifuge tubes? For how long can I use that?
1 answer-
It may be best to store DMSO in amber glass vials for long term storage, as this compound is not compatible with many types of plastics. The compatibility of DMSO with various materials is listed below:
Compatible: LDPE, HDPE, polypropylene, PPCO(polypropylene copolymer), polymethylpentene, nylon, teflon FEP
Moderately compatible: polystyrene, ECTFE/ETFE
Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonateNote that DMSO is hygroscopic and light sensitive. The storage container selected must offer protection from light and moisture uptake. The product is assigned a firm expiration date. Please refer to the lot specific Certificate of Analysis to assume the material is within the expected shelf life.
Helpful?
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E' solo ad uso per ricerca o utilizzabile anche in diagnostica/human? Grazie
1 answer-
This product is not suitable for diagnostic/human use.
Helpful?
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Is this product packaged in a bottle with a septa cap?
1 answer-
No, this product is not packaged with a septa cap. It comes in a 125 ml amber glass bottle with a 28 mm solid white cap.
Helpful?
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Can I use it for PCR?
1 answer-
While this product is tested specifically for use in cell culture, there is no reason to suspect that it would not perform well in PCR applications. The product is NOT tested for amplification, DNAse, or nuclease activity. Should this testing be important, see the link below to product options that include this testing:
D9170 DMSO PCR Reagent:
https://www.sigmaaldrich.com/product/sigma/d9170
D8418 DMSO Molecular Biology Tested
https://www.sigmaaldrich.com/US/en/product/sigma/d8418Helpful?
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