All Photos(2)

D2650

Sigma-Aldrich

Dimethyl sulfoxide

Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Synonym(s):
DMSO
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

Quality Level

grade

Hybri-Max

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

product line

BioReagent

assay

≥99.7%

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

application(s)

cell culture | hybridoma: suitable
cryopreservation: suitable

impurities

endotoxin, tested
≤0.1% water
water

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Packaging

5 mL and 10 mL in flame sealed ampules, 100 mL in amber bottle

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. What is the molarity of DMSO?

    DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

  2. What is the DMSO dissolved in?

    DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

  3. Where does DMSO come from?

    DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

  4. What concentration of DMSO do I use in a cell freezing media?

    DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.

  5. Does DMSO have any synthetic utility?

    DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. What is the specification for 'pass' for cell freezing on the Certificate of Analysis for Dimethyl sulfoxide (DMSO)?

    Product D2438 is assessed for cell freezing.  Cells are frozen and stored at -70°C.  The cells are thawed, cultured and viability is assessed at 24 hours.  There is no evidence of cytotoxicity or abnormal cell growth.

  8. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

  9. What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)?

    The industry standard for 'endotoxin free' is less than 1 EU/mL.

  10. What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?

    DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.

  11. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  12. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  13. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  14. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Azadeh Ghaee et al.
Materials science & engineering. C, Materials for biological applications, 103, 109767-109767 (2019-07-28)
In this study, a simple one-step method was introduced to prepare suitable PCL wound dressing by using a small amount of hydrophilic surface modifying macromolecule (LSMM) with ends capped with Polyethylene glycol and aminated zeolitic imidazolate frameworks (MZIF-8) as a...
Stepan Coufal et al.
Cells, 8(7) (2019-07-25)
Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease (IBD) associated with primary sclerosing cholangitis (PSC-IBD), share three major pathogenetic mechanisms of inflammatory bowel disease (IBD)-gut dysbiosis, gut barrier failure and immune system dysregulation. While clinical differences among them...
L-P Croteau et al.
Scientific reports, 9(1), 12009-12009 (2019-08-21)
Axonal growth cones are guided by molecular cues in the extracellular environment. The mechanisms of combinatorial integration of guidance signals at the growth cone cell membrane are still being unravelled. Limb-innervating axons of vertebrate spinal lateral motor column (LMC) neurons...
Likun Gao et al.
Cell death & disease, 10(8), 609-609 (2019-08-14)
Paeonol (Pae), a phenolic acid compound isolated from the Moutan Cortex, was previously demonstrated to exert multiple anticancer effects. The rational control of autophagy has been considered a potential treatment strategy for epithelial ovarian cancer. However, whether Pae induces autophagy...
Hongxiu Diao et al.
BMC veterinary research, 15(1), 276-276 (2019-08-04)
Mammary gland tumor is the most common spontaneous tumor in intact female dogs, and its poor prognosis remains a clinical challenge. Ivermectin, a well-known anti-parasitic agent, has been implicated as a potential anticancer agent in various types of human cancer....

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Protocols

Trophoblast Protocol

This protocol describes a method for culturing TS cell lines. These cells can then be used to study trophoblast differentiation and placental function.

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service