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D8418

Sigma-Aldrich

Dimethyl sulfoxide

for molecular biology

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Synonym(s):
Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein/REAXYS Number:
506008
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

product line

BioReagent

assay

≥99.9%

form

liquid

autoignition temp.

573 °F

shelf life

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

technique(s)

DNA sequencing: suitable
PCR: suitable
transfection: suitable

impurities

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

absorption

passes test

suitability

suitable for molecular biology

foreign activity

DNase and RNase, none detected

storage temp.

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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41639D2438D9170
Dimethyl sulfoxide for molecular biology

D8418

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41639

Dimethyl sulfoxide

Premium Grade
Dimethyl sulfoxide sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

D2438

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Dimethyl sulfoxide PCR Reagent

D9170

Dimethyl sulfoxide

-
grade

for molecular biology

grade

for molecular biology

grade

BioPerformance Certified

grade

PCR Reagent

Quality Level

200

Quality Level

200

Quality Level

400

Quality Level

200

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

technique(s)

DNA sequencing: suitable, transfection: suitable, PCR: suitable

technique(s)

-

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

PCR: suitable

form

liquid

form

liquid

form

liquid

form

liquid

General description

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Application

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Features and Benefits

This product is free from DNases, RNases, phosphatases and proteases.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Phillip McDonagh et al.
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A Oguro-Ando et al.
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