D8418
Dimethyl sulfoxide
for molecular biology
Synonym(s):
Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO
About This Item
Recommended Products
grade
for molecular biology
Quality Level
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
product line
BioReagent
assay
≥99.9%
form
liquid
autoignition temp.
573 °F
shelf life
Recommended retest period - 2 years
expl. lim.
42 %, 63 °F
technique(s)
DNA sequencing: suitable
PCR: suitable
transfection: suitable
impurities
≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)
color
colorless
refractive index
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: miscible (completely)
density
1.10 g/mL (lit.)
absorption
passes test
suitability
suitable for molecular biology
foreign activity
DNase and RNase, none detected
storage temp.
room temp
SMILES string
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
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General description
- Suitable for Molecular Applications
- Nuclease-Free DMSO
- DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
- Molecular Biology Grade DMSO
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.
Application
- polymerase chain reaction (PCR)
- amplification of cDNA libraries
- DNA sequencing
- column-loading buffers for poly(A)+ RNA selection
- buffers for the transformation of competent E. coli
- transfection protocols.
Dimethyl sulfoxide has been used-
- as an oligonucleotide solvent in array spotting[1]
- as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
- for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
- for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
- in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
Features and Benefits
Caution
also commonly purchased with this product
related product
suggested gloves for splash protection
wgk_germany
WGK 1
flash_point_f
188.6 °F
flash_point_c
87 °C
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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