Skip to Content
MilliporeSigma
Get 22% off for Pi Day through March 31.Save Now

D8418

Dimethyl sulfoxide

Molecular Biology

Dimethyl sulfoxide

Synonym(s):

Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO

Sign In to View Organizational & Contract Pricing.

Select a Size

50 ML

$83.30

100 ML

$147.00

250 ML

$240.00

500 ML

$350.00

1 L

$466.00

$83.30


Check Cart for Availability

Request a Bulk Order
Ships Every 4 weeks

About This Item

Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
UNSPSC Code:
12191502
NACRES:
NA.52
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Assay:
≥99.9%
Grade:
Molecular Biology
Technique(s):
DNA sequencing: suitable, PCR: suitable, transfection: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

grade

Molecular Biology

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

product line

BioReagent

assay

≥99.9%

form

liquid

autoignition temp.

573 °F

shelf life

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

technique(s)

DNA sequencing: suitable, PCR: suitable, transfection: suitable

impurities

≤0.001 meq/g Titratable acid, ≤0.1% water (Karl Fischer)

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

absorption

passes test

suitability

suitable for molecular biology

foreign activity

DNase and RNase, none detected

storage temp.

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
41639D9170PHR1309
assay

≥99.9%

assay

≥99.5% (GC)

assay

≥99.5%

assay

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

grade

for molecular biology

grade

for molecular biology

grade

PCR Reagent

grade

certified reference material, pharmaceutical secondary standard

technique(s)

DNA sequencing: suitable, transfection: suitable, PCR: suitable

technique(s)

-

technique(s)

PCR: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

-

form

liquid

form

liquid

form

liquid

form

-

General description

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Application

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Features and Benefits

This product is free from DNases, RNases, phosphatases and proteases.

Disclaimer

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many
Caroline Godfrey et al.
Human molecular genetics, 24(15), 4225-4237 (2015-05-03)
Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle
Phillip McDonagh et al.
Veterinary microbiology, 174(3-4), 438-447 (2014-12-04)
Feline infectious peritonitis (FIP), a feline coronavirus (FCoV) induced disease, is almost invariably fatal with median life expectancy measured in days. Current treatment options are, at best, palliative. The objectives of this study were to evaluate a panel of nineteen
Claudia C Faria et al.
Cancer research, 75(1), 134-146 (2014-11-14)
Medulloblastoma is the most common malignant pediatric brain tumor, with metastases present at diagnosis conferring a poor prognosis. Mechanisms of dissemination are poorly understood and metastatic lesions are genetically divergent from the matched primary tumor. Effective and less toxic therapies
Mike Clements et al.
Toxicological sciences : an official journal of the Society of Toxicology, 140(2), 445-461 (2014-05-09)
Human stem cell derived cardiomyocytes (hESC-CM) provide a potential model for development of improved assays for pre-clinical predictive drug safety screening. We have used multi-electrode array (MEA) analysis of hESC-CM to generate multi-parameter data to profile drug impact on cardiomyocyte

Articles

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.

Protocols

Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Related Content

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

Product Information Sheet

Global Trade Item Number

SKUGTIN
D8418-1L04061837896873
D8418-500ML04054839596322
D8418-1VL04061837299919
D8418-5.5ML04061837299926
D8418-1.5ML04061837299896
D8418-7.5ML04061837299933
D8418-1ML04061837299902
D8418-250ML04061833589618
D8418-100ML04061837896866
D8418-50ML04061837896880

Questions

1–10 of 19 Questions  
  1. Is this product anhydrous?

    1 answer
    1. This product is not anhydrous. The maximum allowable water content is < 0.1%. Please see the link below to review an anhydrous product option.
      276855
      https://www.sigmaaldrich.com/product/sial/276855

      Helpful?

  2. May I know the concentration (mg/ml) for DMSO (cat no. D8418)?

    1 answer
    1. This product is supplied as a neat or pure liquid with a density of 1.10 g/mL and a molecular weight of 78.13 g/mol. The minimum purity is 99.9%, thus the molarity will vary slightly from lot to lot. The molarity is 14.1 M.

      Helpful?

  3. Is Product D8418, Dimethyl sulfoxide, also compatible with PET material?

    1 answer
    1. Yes, Dimethyl sulfoxide (DMSO) is generally considered to be compatible with Polyethylene terephthalate (PET) material. The following information can be found on the Product Data Sheet:

      -Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, teflon FEP
      -Moderately compatible: polystyrene, ECTFE/ETFE
      -Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265/d2650pis.pdf

      Helpful?

  4. Is this product sterile?

    1 answer
    1. This product is not sterile. To prepare a sterile filtered DMSO solution, it is recommended to use a Teflon, polypropylene, or nylon membrane. See products D2650 or D2438 for sterile DMSO product options. Please review these products at the links provided below:
      https://www.sigmaaldrich.com/product/sigma/d2650
      https://www.sigmaaldrich.com/product/sigma/d2438

      Helpful?

  5. I received Product D8418, Dimethyl sulfoxide, as a frozen solid. But isn't it supposed to be in the liquid state?

    1 answer
    1. DMSO super cools easily and remelts slowly at room temperature. The product may arrive as a solid instead of a liquid. The solidified product can be reliquified by warming to room temperature without detriment to the product.

      Helpful?

  6. What is the molarity of DMSO?

    1 answer
    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  7. What is the DMSO dissolved in?

    1 answer
    1. DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

      Helpful?

  8. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    1 answer
    1. DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

      Helpful?

  9. Is Dimethyl sulfoxide a sterile product?

    1 answer
    1. No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

1–10 of 19 Questions  

Reviews

Active Filters

    • Review 1
    • Votes 4
    5 out of 5 stars.

    DMSO

    it is good for solvent and we use it for long time , it is pure ans stable

    Helpful?

  1. 2 Ratings-Only Reviews

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service