R1755

Rhodamine B isothiocyanate

BioReagent, mixed isomers, suitable for protein labeling

• Synonym(s): RBITC
• Empirical Formula (Hill Notation): C₂₉H₃₀ClN₃O₃S
• CAS Number: 36877-69-7
• Molecular Weight: 536.08
• NACRES: NA.32
• PubChem Substance ID: 329824007
• UNSPSC Code: 12352116
• EC Number: 253-248-9
• MDL number: MFCD00136007

Properties

• Product Name: Rhodamine B isothiocyanate, BioReagent, mixed isomers, suitable for protein labeling
• InChI key: YVSWPCCVTYEEHG-UHFFFAOYSA-N
• InChI: 1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H
• SMILES string: [Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1
• description: labeling efficiency with bovine albumin > 70%
• product line: BioReagent
• Quality Level: 200
• form: powder
• mol wt: 536.08 g/mol
• composition: 9-[2-carboxy-5(or 6)-isothiocyanatophenyl]-3,6-bis(diethylamino)xanthylium chloride, ≤100%
• color: purple to red-brown
• solubility: methanol: 10 mg/mL (dark red to very dark red and dark purple to very dark purple)
• fluorescence: λ_ex 543 nm; λ_em 580 nm in methanol
• suitability: suitable for protein labeling
• storage temp.: −20°C

Description

Application

Rhodamine B isothiocyanate is used for the following applications:

• Rhodamine isothiocyanate labeling
• Rhodamine-labeled synthetic APF
• Preparations of protein and fluorescent conjugates
• Fluorescent red dye

General description

Rhodamine B isothiocyanate (RhBITC) is an organic fluorescence dye and is widely used as a fluorescent tag in a variety of biological applications. RhBITC is adsorbed onto Ag on silica or polystyrene beads. It exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol. They are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H302 + H312 + H332,H315,H319,H334,H335
• pcodes: P261 - P264 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves