324865

PhosphoDetect Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb

liquid, Calbiochem^®

• 别名: Anti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)
• 分類程式碼代碼: 12352203
• NACRES: NA.43

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: liquid
• 包含: ≤0.1% sodium azide as preservative
• 物種活性: human
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (1:1000))
• 同型: IgG
• 運輸包裝: wet ice
• 儲存溫度: −70°C
• 目標翻譯後修改: phosphorylation (pTyr845)
• 基因資訊: human ... EGFR(1956)

说明

一般說明

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic tyrosine phosphorylated peptide, followed by immunoaffinity chromatography. Recognizes the ~185 KDa EGFR protein phosphorylated on Tyr⁸⁴⁵.

Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr⁸⁴⁵ in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

This PhosphoDetect Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr⁸⁴⁵).

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR

應用

Immunoblotting (1:1000)

外觀

In Dulbecco′s PBS (w/o Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.4.

準備報告

Following initial thaw, aliquot and freeze (-70°C).

分析報告

Positive Control
A431 cells stimulated with EGF

其他說明

Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem.274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.

The immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Toxicity: Standard Handling (A)

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable