407615-M

Interleukin-1β, Human, Recombinant, E. coli

• 别名: Interleukin-1β, Human, Recombinant, E. coli, rhIL-1β
• 化学文摘社编号: 97599-21-8
• MDL號碼: MFCD00064956
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• 描述: Merck USA index - 14, 4993
• 品質等級: 100
• 化驗: ≥97% (SDS-PAGE)
• 形狀: lyophilized
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 雜質: ≤1.0 EU/μg Endotoxin (EU/μg IL-1β)
• 儲存溫度: −70°C

说明

一般說明

Recombinant, human interleukin-1β expressed in E. coli. Major form of interleukin-1 secreted by monocytes and macrophages. IL-1β is activated by proteolytic cleavage of the precursor molecule. Increases nitric oxide production in smooth muscle cells and suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing. Induces nitric oxide synthase in insulin-producing cells. 1 µg of IL-1β corresponds to 1.9 x 10⁵ WHO units.

生化／生理作用

ED₅₀ = <12 pg/ml as measured in a cell proliferation assay using the murine T-cell line, D10.G4.1 (Symons, J.A. et al. 1987)

外觀

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.

準備報告

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

其他說明

Chun, S.Y., et al. 1995. Endocrinology136, 3120.
Kunz, D., et al. 1994. Biochem. J. 304, 337.
Eizirik, D.L., et al. 1993. FEBS Lett. 317, 62.
Dinarello, C>A> 1991. Blood77, 1627.
Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun.162, 357.
Symons, J.A., et al. 1987. In Lymphokines and interferons, A Practical Approach (Clemens, M.J., et al. eds.) IRL Press, Oxford p. 272.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Toxicity: Standard Handling (A)

安全信息

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable