AB5970

Anti-Dishevelled-1 Antibody

Chemicon^®, from rabbit

• 别名: DSH, DVL1, DSH Homolog 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 共軛: unconjugated
• 抗體表格: affinity purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 製造商／商標名: Chemicon^®
• 技術: western blot: suitable
• 輸入: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• NCBI登錄號: NM_004421.2; NM_181870.1; NM_182779.3
• UniProt登錄號: O14640
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... DVL1(1855)

说明

一般說明

85 kDa

DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation. The protein acts as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 mutation is likely responsible for transformation in neuroblastomas, and the Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1 gene, thus DVL1 maybe important in these diseases as well.

免疫原

Synthetic peptide corresponding to amino acids 557-571 of human dishevelled-1 protein.

應用

Anti-Dishevelled-1 Antibody is an antibody against Dishevelled-1 for use in WB.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells

Western blot: 1:500 using ECL. The antibody reacts with a protein of ~85kDa. Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

生化／生理作用

Dishevelled-1 protein (dvl-1). By Western blot the antibody shows no cross reactivity with recombinant dishevelled -2 or dishevelled -3 protein Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against dishevelled -1 protein.

外觀

Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

準備報告

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

分析報告

Control
SK-N-SH (neuroblastoma) whole cell lysates

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable